Nomoto S, Muramatsu H, Ozawa M, Suganuma T, Tashiro M, Muramatsu T
Exp Cell Res. 1986 May;164(1):49-62. doi: 10.1016/0014-4827(86)90453-2.
A monoclonal rat IgM antibody (4C9) raised against F9 embryonal carcinoma cells reacted with fucosyl residues in poly-N-acetyllactosamine-type large carbohydrates of these cells (embryoglycan). The chemical properties and distribution of the antigen resembled those of SSEA-1. The monoclonal antibody was found to inhibit cell-substratum adhesion of F9 cells: in the presence of the antibody, cells grew as spherical cell aggregates on plastic dishes. When the antibody was added to the already spread cells, they displayed the initial sign of rounding up within 3 h; the rounding process was largely completed within 6 h. After removal of the antibody, cells resumed their normal morphology. The antibody could act in the presence of 2,4-dinitrophenol. In serum-free medium, F9 cells spread on plastic dishes coated with fibronectin or with laminin, and the process was also inhibited by the antibody. Immuno-electronmicroscopy revealed that 4C9 antigen was diffusely distributed over the cell surface of F9 cells. The distribution of the antigen was not altered generally after culturing with the antibody for 6 h. Another monoclonal rat IgM antibody, which did not react with embryoglycan and resembled anti-Forssman, did not inhibit cell-substratum adhesion of F9 cells, in spite of its reactivity to the cells. Thus, a glycoprotein with fucosyl (poly)-N-acetyllactosamine structure appears to be involved in cell-substratum adhesion of F9 cells.
一种针对F9胚胎癌细胞产生的大鼠单克隆IgM抗体（4C9）与这些细胞（胚胎聚糖）的多聚N-乙酰乳糖胺型大碳水化合物中的岩藻糖基残基发生反应。该抗原的化学性质和分布与阶段特异性胚胎抗原-1（SSEA-1）相似。发现该单克隆抗体可抑制F9细胞与底物的黏附：在抗体存在的情况下，细胞在塑料培养皿上呈球形细胞聚集体生长。当将抗体添加到已经铺展的细胞中时，它们在3小时内显示出开始变圆的迹象；变圆过程在6小时内基本完成。去除抗体后，细胞恢复其正常形态。该抗体在2,4-二硝基苯酚存在的情况下仍可发挥作用。在无血清培养基中，F9细胞可铺展在包被有纤连蛋白或层粘连蛋白的塑料培养皿上，且该过程也受到抗体的抑制。免疫电子显微镜显示，4C9抗原在F9细胞的细胞表面呈弥漫性分布。在用抗体培养6小时后，抗原的分布总体上没有改变。另一种不与胚胎聚糖反应且类似于抗福斯曼抗体的大鼠单克隆IgM抗体，尽管其与细胞有反应性，但并未抑制F9细胞与底物的黏附。因此，一种具有岩藻糖基（多）-N-乙酰乳糖胺结构的糖蛋白似乎参与了F9细胞与底物的黏附。
