Rule P L, Alexander A D
J Clin Microbiol. 1986 Mar;23(3):500-4. doi: 10.1128/jcm.23.3.500-504.1986.
An albumin polysorbate semisolid medium (Ellinghausen McCullough Johnson Harris medium) gelled with gellan gum (Gelrite; Kelco Div., Merck & Co., Inc.) compared favorably with conventional agar media for the cultivation of both pathogenic and saprophytic leptospires. The gellan gum medium supported the growth of all 18 leptospiral strains studied which included an array of serovars with various fastidious growth characteristics. Gellan gum medium was also used advantageously as a long-term maintenance medium; 9- to 12-month-old cultures still contained viable organisms. The colonial growth in gellan gum plating medium of six representative strains was consistent with previously described colonial growth on agar plating media. In addition, gellan gum medium appeared to be an excellent medium for the recovery of leptospires from the blood, liver, and kidneys of hamsters experimentally infected with a virulent Leptospira interrogans serovar bataviae strain. As few as 1 to 10 organisms in the infective tissue could be recovered in semisolid Ellinghausen McCullough Johnson Harris-gellan gum medium. The antigenicity did not appear to be affected by growth in gellan gum medium. The hamster-virulent strain of L. interrogans serovar bataviae isolated from a moribund hamster maintained its virulence after 10 sequential passages in gellan gum medium. Gellan gum medium can be a valuable adjunct to currently used cultural procedures.
一种用结冷胶（杰丽特；凯尔科分公司，默克公司）胶凝的白蛋白聚山梨醇酯半固体培养基（埃林豪森 - 麦卡洛 - 约翰逊 - 哈里斯培养基），在培养致病性和腐生性钩端螺旋体方面，与传统琼脂培养基相比具有优势。结冷胶培养基支持所研究的全部18种钩端螺旋体菌株的生长，这些菌株包括一系列具有各种苛求生长特性的血清型。结冷胶培养基还可有效地用作长期维持培养基；9至12个月大的培养物中仍含有活的生物体。六种代表性菌株在结冷胶平板培养基上的菌落生长情况与先前描述的在琼脂平板培养基上的菌落生长情况一致。此外，结冷胶培养基似乎是从实验感染强毒问号钩端螺旋体巴达维亚血清型菌株的仓鼠血液、肝脏和肾脏中分离钩端螺旋体的优良培养基。在半固体埃林豪森 - 麦卡洛 - 约翰逊 - 哈里斯 - 结冷胶培养基中，感染组织中少至1至10个生物体都能被分离出来。抗原性似乎不受在结冷胶培养基中生长的影响。从濒死仓鼠分离出的问号钩端螺旋体巴达维亚血清型仓鼠强毒株在结冷胶培养基中连续传代10次后仍保持其毒力。结冷胶培养基可以成为当前使用的培养程序的有价值的辅助手段。
