Biotechnology Group Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box: 14115-111, Tehran, the Islamic Republic of Iran; Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965-161, Tehran, the Islamic Republic of Iran.
Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965-161, Tehran, the Islamic Republic of Iran.
Colloids Surf B Biointerfaces. 2023 Oct;230:113487. doi: 10.1016/j.colsurfb.2023.113487. Epub 2023 Aug 1.
Protein fibrillation is a phenomenon associated with misfolding and the production of highly ordered nanofibrils, which may cause serious degenerative diseases such as Parkinson's disease, Alzheimer's disease, and type 2 diabetes. Upon contact with biological fluids, the nanomaterials are immediately covered by proteins and interact with them. In this study, the effects of Graphene NanoPlateles (Plain-GNPs) and their modified forms with a carboxyl group (GNPs -COOH) and an amine group (GNPs -NH) are evaluated on the fibrillation process of Hen Egg White Lysozyme (HEWL). The fibrillation process of HEWL was studied using thioflavin-T, Circular Dichroism spectrometry, and Atomic Force Microscopy. Plain-GNPs significantly decreased the fibrillation process at different stages, including nucleation, exponential fibrillation phases, and end-mature fibril products. However, GNPs-COOH and GNPs-NH affected the final fluorescence of ThT. The species formed in the presence of Plain-GNPs showed less toxicity in SH-SY5Y cells, which could be applicable for therapeutic purposes.
蛋白质纤维形成是一种与错误折叠和产生高度有序的纳米纤维有关的现象，可能导致严重的退行性疾病，如帕金森病、阿尔茨海默病和 2 型糖尿病。纳米材料与生物流体接触时，会立即被蛋白质覆盖并与之相互作用。在这项研究中，评估了 Graphene NanoPlateles（普通-GNPs）及其带有羧基（GNPs-COOH）和氨基（GNPs-NH）的修饰形式对 Hen Egg White Lysozyme（HEWL）纤维形成过程的影响。使用硫黄素 T、圆二色性光谱法和原子力显微镜研究了 HEWL 的纤维形成过程。普通-GNPs 显著降低了纤维形成过程的不同阶段，包括成核、指数增长纤维阶段和成熟终产物纤维。然而，GNPs-COOH 和 GNPs-NH 影响了 ThT 的最终荧光。在 Plain-GNPs 存在下形成的物质在 SH-SY5Y 细胞中的毒性较小，可适用于治疗目的。
