Center for Biospecimen Research & Development, New York University Grossman School of Medicine, New York, NY, 10016, USA.
Center for Biospecimen Research & Development, New York University Grossman School of Medicine, New York, NY, 10016, USA.
Anal Biochem. 2023 Oct 1;678:115271. doi: 10.1016/j.ab.2023.115271. Epub 2023 Aug 3.
With the development of genomic technologies, the isolation of genomic DNA (gDNA) from clinical samples is increasingly required for clinical diagnostics and research studies. In this study, we explored the potential of utilizing various leftover blood samples obtained from routine clinical tests as a viable source of gDNA. Using an automated method with optimized pre-treatments, we obtained gDNA from seven types of clinical leftover blood, with average yields of gDNA ranging from 3.11 ± 0.45 to 22.45 ± 4.83 μg. Additionally, we investigated the impact of storage conditions on gDNA recovery, resulting in yields of 8.62-68.08 μg when extracting gDNA from EDTA leftover blood samples stored at 4 °C for up to 13 weeks or -80 °C for up to 78 weeks. Furthermore, we successfully obtained sequenceable gDNA from both Serum Separator Tube and EDTA Tube using a 96-well format extraction, with yields ranging from 0.61 to 71.29 μg and 3.94-215.98 μg, respectively. Our findings demonstrate the feasibility of using automated high-throughput platforms for gDNA extraction from various clinical leftover blood samples with the proper pre-treatments.
随着基因组技术的发展，从临床样本中分离基因组 DNA（gDNA）越来越需要用于临床诊断和研究。在这项研究中，我们探索了利用各种常规临床测试获得的剩余血液样本作为 gDNA 有效来源的潜力。使用经过优化预处理的自动化方法，我们从七种类型的临床剩余血液中获得了 gDNA，gDNA 的平均产量范围为 3.11±0.45 至 22.45±4.83μg。此外，我们研究了储存条件对 gDNA 回收的影响，从储存于 4°C 长达 13 周或 -80°C 长达 78 周的 EDTA 剩余血液样本中提取 gDNA 时，产量为 8.62-68.08μg。此外，我们使用 96 孔格式提取，从血清分离管和 EDTA 管中成功获得了可测序的 gDNA，产量分别为 0.61-71.29μg 和 3.94-215.98μg。我们的研究结果表明，使用适当的预处理，自动化高通量平台从各种临床剩余血液样本中提取 gDNA 是可行的。
