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氧化铁纳米粒子与硫代卡巴肼缀合通过增加肺癌 A549 细胞中 的基因表达来减少癌细胞的存活。

Iron Oxide Nanoparticles Conjugated to Thiosemicarbazone Reduce the Survival of Cancer Cells by Increasing the Gene Expression of in Lung Cancer A549 Cells.

机构信息

Departman of Basic Sciences, Faculty of Science, Imam Hossein University, Tehran, Iran.

Departman of Molecular Cell Biology, Faculty of Science, Islamic Azad University of Lahijan, Lahijan, Iran.

出版信息

Arch Iran Med. 2022 Dec 1;25(12):807-816. doi: 10.34172/aim.2022.126.

DOI:10.34172/aim.2022.126
PMID:37543908
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10685841/
Abstract

BACKGROUND

Cancer cells have a higher demand for iron to grow and proliferate. A new complex of iron nanoparticles and thiosemicarbazones was synthesized. Confirmation tests included UV-visible, scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), Fourier transform infrared (FTIR), X-ray diffraction (XRD) and zeta potential.

METHODS

MTT assay, flow cytometry and qRT-PCR were used to investigate anti-proliferative effect, amount of apoptosis and the effect of Fe O @Glu/BTSC on changes in gene expression of (), respectively. The specifications of Fe O @ Glu/BTSC were confirmed at 5 nm.

RESULTS

Fe3O4@Glu/BTSC was more effective than BTSC and Fe O on A549 cells (IC=166.77 µg/mL) but its effect on healthy cells was smaller (CC=189.15 µg/mL). The drug selectivity index (SI) was calculated to be 1.13. The initial apoptosis rate was 46.33% for Fe O @Glu/BTSC, 28.27% for BTSC and 26.02% for Fe O . BTSC and BTSC@Fe O inhibited the cell cycle progression in the Sub-G1 and S phases. expression was 6.9 times higher in treated cells compared to the control group. The expression rate was 2.2 with BTSC compared to the control group and 1.6 times for Fe O.

CONCLUSION

Fe O @Glu/BTSC has proper anti-proliferative effects against lung cancer cells by increasing the expression of and inhibiting the cell cycle with the apoptosis activation pathway.

摘要

背景

癌细胞生长和增殖对铁的需求更高。合成了一种新型的铁纳米粒子和硫代氨基甲酸盐复合物。确认测试包括紫外-可见分光光度法、扫描电子显微镜（SEM）、能谱分析（EDX）、傅里叶变换红外（FTIR）、X 射线衍射（XRD）和zeta 电位。

方法

MTT 测定法、流式细胞术和 qRT-PCR 分别用于研究抗增殖作用、凋亡程度以及 Fe O @Glu/BTSC 对 （）基因表达变化的影响。Fe O @Glu/BTSC 的规格被确认为 5nm。

结果

Fe3O4@Glu/BTSC 对 A549 细胞的作用比 BTSC 和 Fe O 更有效（IC=166.77µg/mL），但其对健康细胞的作用较小（CC=189.15µg/mL）。药物选择性指数（SI）计算为 1.13。Fe O @Glu/BTSC 的初始凋亡率为 46.33%，BTSC 为 28.27%，Fe O 为 26.02%。BTSC 和 BTSC@Fe O 抑制细胞周期在 Sub-G1 和 S 期的进展。与对照组相比，处理细胞中的 表达增加了 6.9 倍。与对照组相比，BTSC 的表达率为 2.2，Fe O 为 1.6 倍。

结论

Fe O @Glu/BTSC 通过增加 的表达并通过凋亡激活途径抑制细胞周期，对肺癌细胞具有适当的抗增殖作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea4d/10685841/5aea23c63c12/aim-25-807-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea4d/10685841/a673725baa26/aim-25-807-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea4d/10685841/2e8e4b683fd2/aim-25-807-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea4d/10685841/986f6b8c512f/aim-25-807-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea4d/10685841/abcfc22b5810/aim-25-807-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea4d/10685841/5aea23c63c12/aim-25-807-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea4d/10685841/a673725baa26/aim-25-807-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea4d/10685841/5b12428a1054/aim-25-807-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea4d/10685841/2e8e4b683fd2/aim-25-807-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea4d/10685841/986f6b8c512f/aim-25-807-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea4d/10685841/abcfc22b5810/aim-25-807-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea4d/10685841/5aea23c63c12/aim-25-807-g010.jpg

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