State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, School of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, Zhejiang, PR China.
Hangzhou Pupai Technology Co., Ltd, Hangzhou 310000, China.
Food Chem. 2024 Jan 1;430:137049. doi: 10.1016/j.foodchem.2023.137049. Epub 2023 Aug 1.
The ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS) method was built to quantify the casein glycomacropeptide (CGMP) in bovine dairy products accurately based on targeted proteomics. Qualitative analysis of theoretical peptides was carried out using high-resolution mass spectrometry (HRMS) and protein software. Isotope-labeled characteristic peptides were acquired via the labeled amino acid condensation method to correct the matrix effects. Peptide MAIPPK was the representative characteristic peptide for distinguishing the CGMP from κ-casein through trypsin digestion. After optimizing the pre-treatment conditions, the final 8% oxidant concentration was selected and the 10% formic acid concentration with 2.5 h oxidation time. Moreover, the results of methodological verification showed that the recovery rate was 103.7%, meanwhile the precision of inter-day and intra-day was less than 5%. In conclusion, the research demonstrated the characteristic peptide MAIPPK could quantitatively applied to detect CGMP in dairy products.
基于靶向蛋白质组学，建立了超高效液相色谱串联质谱（UHPLC-MS）法，以准确定量牛乳制品中的酪蛋白糖巨肽（CGMP）。采用高分辨质谱（HRMS）和蛋白质软件对理论肽进行定性分析。通过标记氨基酸缩合的方法获得同位素标记的特征肽，以校正基质效应。肽 MAIPPK 是通过胰蛋白酶消化区分 CGMP 和 κ-酪蛋白的代表性特征肽。在优化预处理条件后，最终选择 8%的氧化剂浓度和 10%甲酸浓度，氧化时间为 2.5 h。此外，方法验证结果表明，回收率为 103.7%，日内和日间精密度均小于 5%。总之，该研究表明特征肽 MAIPPK 可定量应用于乳制品中 CGMP 的检测。
