School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China; State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University), Guilin, 541004, China; Key Laboratory of Medical Electrophysiology, Ministry of Education, Institute of Cardiovascular Research of Southwest Medical University, Luzhou, Sichuan, 646000, China.
J Chromatogr A. 2023 Sep 13;1706:464268. doi: 10.1016/j.chroma.2023.464268. Epub 2023 Aug 2.
Poly(ethyleneimine) (PEI) modified silica spheres were used to graft butyric acid and octanoic acid onto their surfaces, forming two stationary phases named Sil-PEI-BAD and Sil-PEI-CAD, respectively. Characterized methods including fourier transform infrared spectroscopy (FT-IR), elemental analysis (EA) and thermogravimetric analysis (TGA) were utilized to determine the successful synthesis of these two stationary phase materials. The chromatographic performance of these two stationary phases was analyzed with hydrophobic and hydrophilic compounds as analytes. Compared with Sil-PEI-CAD column, Sil-PEI-BAD column was more effective in separating hydrophilic compounds including nucleosides, alkaloids and vitamins. Hydrophobic substances including polycyclic aromatic hydrocarbons (PAHs) and alkylbenzenes obtained excellent separation results on Sil-PEI-CAD column than Sil-PEI-BAD column. Additionally, according to the separation of phenols, Sil-PEI-CAD column can be used in HILIC/RPLC mixed-mode. The results showed that the properties and retention mechanisms of the prepared stationary phases depended on the length of the alkyl chains bonded on the silica surface.
聚（乙二胺）（PEI）修饰的硅胶球被用于将丁酸和辛酸接枝到其表面，形成两种固定相，分别命名为 Sil-PEI-BAD 和 Sil-PEI-CAD。利用傅里叶变换红外光谱（FT-IR）、元素分析（EA）和热重分析（TGA）等特征方法确定了这两种固定相材料的成功合成。利用疏水性和亲水性化合物作为分析物来分析这两种固定相的色谱性能。与 Sil-PEI-CAD 柱相比，Sil-PEI-BAD 柱在分离包括核苷、生物碱和维生素在内的亲水性化合物方面更有效。Sil-PEI-CAD 柱对包括多环芳烃（PAHs）和烷基苯在内的疏水性物质的分离效果优于 Sil-PEI-BAD 柱。此外，根据酚类物质的分离情况，Sil-PEI-CAD 柱可用于 HILIC/RPLC 混合模式。结果表明，所制备的固定相的性能和保留机制取决于键合在硅胶表面的烷基链的长度。
