Anhui Engineering Laboratory for Agro-Products Processing, School of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Anhui Engineering Laboratory for Agro-Products Processing, School of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Int J Biol Macromol. 2023 Sep 30;249:126141. doi: 10.1016/j.ijbiomac.2023.126141. Epub 2023 Aug 4.
This study investigated the effects of pullulanase debranching on the structural properties and digestibility of maize starch (MS)-glyceryl monostearate (GMS) complexes. According to our results, the apparent amylose content of MS increased from 36.34 % to 95.55 % and complex index reached 93.09 % after 16 h of pullulanase debranching. The crystallinity of prepared MS-GMS complexes increased to 33.24 % with a blend of B-type and V-type crystals. The surface of prepared MS-GMS complexes granules emerged more small lamellar crystals tightly adhering to the surface of granules. The Fourier transforms infrared spectroscopy analysis showed that debranching pretreatment MS-GMS complexes exhibited higher levels of short-range orders structure. These results indicated that maize starch was favorable to form more ordered starch-lipid complexes structure after debranching pretreatment, which resulted in the restriction of starch hydrolysis. In vitro digestion data implied that resistant starch (RS) content increased with the extension of the debranching time, and the highest RS content (69.58 %) appeared with 16 h pullulanase debranching. This work suggests that debranching pretreatment could be an efficient way to produce ordered starch-lipid complexes with controllable structure and anti-digestibility.
本研究考察了普鲁兰酶解支对玉米淀粉（MS）-甘油单硬脂酸酯（GMS）复合物结构性质和消化性的影响。根据我们的结果，经过 16 小时的普鲁兰酶解支后，MS 的直链淀粉含量从 36.34%增加到 95.55%，复合物指数达到 93.09%。制备的 MS-GMS 复合物的结晶度增加到 33.24%，形成了 B 型和 V 型晶体的混合物。制备的 MS-GMS 复合物颗粒表面出现了更多紧密附着在颗粒表面的小片状晶体。傅里叶变换红外光谱分析表明，解支预处理 MS-GMS 复合物表现出更高水平的短程有序结构。这些结果表明，玉米淀粉在解支预处理后有利于形成更有序的淀粉-脂质复合物结构，从而限制了淀粉的水解。体外消化数据表明，抗性淀粉（RS）含量随着解支时间的延长而增加，16 小时普鲁兰酶解支时出现最高的 RS 含量（69.58%）。这项工作表明，解支预处理可以是一种生产具有可控结构和抗消化性的有序淀粉-脂质复合物的有效方法。
