Department of Medical Biology and Central Electron Microscopy Laboratory, Medical School, Pecs University, Pecs, Hungary.
Szentágothai János Research Center, Pecs, Hungary.
Front Immunol. 2023 Jul 21;14:1200704. doi: 10.3389/fimmu.2023.1200704. eCollection 2023.
The evidence concerning the role of vitamin D (VD) in reproduction is still inconclusive. Calcitriol was given to superovulated female mice at the time of FSH injection (Group A), or at day 0.5 of pregnancy (Group B). The retrieved and cultured embryos were transferred to the uteri of pseudopregnant females. Ten animals from each group conceived naturally, and at day 7.5 of pregnancy, the implantation sites were counted. Serum hormone concentrations were determined by ELISA. The expression of CD70, PD-L1, OX-40L, and PIBF on extracellular vesicles (EVs) was tested by flow cytometry. Calcitriol treatment did not alter serum oestradiol concentrations, while 25(OH) D levels significantly decreased in both treated groups. Progesterone concentrations were significantly higher in group A and lower in group B than in the controls. On EVs produced by group B embryos PIBF, CD70, and OX-40L expression were significantly lower, while that of PD-L1 was significantly higher than that of controls. Calcitriol treatment decreased the fertilization rate in group A, and the blastulation rate of cultured embryos in group B, while the implantation capacity of the embryos was not affected, suggesting that depending on the time of administration, VD has an adverse effect on oocyte maturation and embryo development, but not on the implantation rates.
关于维生素 D（VD）在生殖中的作用的证据仍然不确定。在 FSH 注射时（A 组）或妊娠第 0.5 天（B 组）给超排卵雌性小鼠给予钙三醇。回收和培养的胚胎被转移到假孕雌性的子宫中。每组 10 只动物自然受孕，在妊娠第 7.5 天，计算着床部位。通过 ELISA 测定血清激素浓度。通过流式细胞术测试细胞外囊泡（EVs）上的 CD70、PD-L1、OX-40L 和 PIBF 的表达。钙三醇处理不会改变血清雌二醇浓度，而两组处理组的 25(OH)D 水平均显著降低。与对照组相比，A 组的孕酮浓度显著升高，B 组的孕酮浓度显著降低。在 B 组胚胎产生的 EVs 上，PIBF、CD70 和 OX-40L 的表达明显降低，而 PD-L1 的表达明显高于对照组。钙三醇处理降低了 A 组的受精率，B 组培养胚胎的囊胚形成率，而胚胎的着床能力不受影响，这表明 VD 的作用取决于给药时间，对卵母细胞成熟和胚胎发育有不利影响，但对着床率没有影响。
