Cohen J, Somma-Delpero C, Verine A, Codaccioni J L, Boyer J
J Endocrinol. 1986 Mar;108(3):357-9. doi: 10.1677/joe.0.1080357.
Human red blood cells (RBC) contain a monoester lipase (MEL) activity which is tightly associated with the cell membrane and has its active site externally oriented, as inferred from the ability of the intact cell to hydrolyse an exogenously added lipid substrate. Membrane-bound MEL activity was measured by a radiochemical assay in intact RBC from 29 untreated hyperthyroid patients. The mean (+/- S.D.) MEL level was higher (P less than 0.01) in these patients (1220 +/- 212 mu./10(12) RBC) than in the control group (1010 +/- 120 mu./10(12) RBC). There was no difference between men and women. The increase in MEL values was associated with significantly (P less than 0.001) decreased values of mean cellular volume and mean cellular haemoglobin content. A continued study of 13 patients, who became euthyroid with treatment, showed a normalization of the MEL values in RBC. The increased lipolytic potency of RBC represents a new characteristic of hyperthyroid patients. Further exploration of the possible diagnostic or prognostic implications of this enzymatic change seems warranted.
人类红细胞（RBC）含有一种单酯脂肪酶（MEL）活性，该活性与细胞膜紧密相关，且其活性位点朝外，这是根据完整细胞水解外源性添加脂质底物的能力推断出来的。通过放射化学分析法测定了29名未经治疗的甲状腺功能亢进患者完整红细胞中膜结合的MEL活性。这些患者（1220±212 μ/10¹²个红细胞）的平均（±标准差）MEL水平高于对照组（1010±120 μ/10¹²个红细胞）（P＜0.01）。男性和女性之间无差异。MEL值的增加与平均细胞体积和平均细胞血红蛋白含量的显著降低（P＜0.001）相关。对13名经治疗后甲状腺功能恢复正常的患者进行的持续研究表明，红细胞中的MEL值恢复正常。红细胞脂解能力的增强是甲状腺功能亢进患者的一个新特征。似乎有必要进一步探索这种酶变化可能的诊断或预后意义。
