Matsubara Takeshi, Ishikawa Fuka, Inuo Chisato, Fujita Mayumi, Tsukahara Ayumi, Koyama Takahiro, Iwamoto Hiroshi, Miyaji Kazuhiro
Health Care & Nutrition Science Institute, R&D Section, Morinaga Milk Industry Co., Ltd., Zama, Kanagawa, Japan.
Department of Allergy, Kanagawa Children's Medical Center, Yokohama, Kanagawa, Japan.
Front Allergy. 2023 Jul 21;4:1207924. doi: 10.3389/falgy.2023.1207924. eCollection 2023.
When exclusive breastfeeding is not possible, partially hydrolyzed formula (PHF) is often used as a starter formula for infants. Some children develop allergic symptoms, including anaphylaxis, after the first intake of cow protein. Therefore, the tolerability of PHF in infants with cow's milk allergy (CMA) is important information. Partially hydrolyzed whey formula (PHWF) is well characterized, but those containing both whey and casein are also available. We evaluated the characteristics of two whey and casein PHFs, PHF1 and PHF2, and , and compared them with a PHWF, PHWF1.
Residual antigenicity of β-lactoglobulin (β-LG) and casein in the formulas was measured using ELISA. The molecular weight profile was determined using high-pressure liquid chromatography. IgE reactivity and allergenic activity of the formulas were evaluated by ImmunoCAP inhibition assay and by basophil activation test using blood from patients with CMA, respectively.
All the participants ( = 10) had casein-specific IgE. The antigenicity of β-LG in PHF1 was similar to that in PHWF1, but it was slightly higher than that in PHWF1 for casein. PHF1 had a higher IgE reactivity than PHWF1. However, PHF1 and PHWF1 had a similar ability to activate basophils. PHF2 had lower antigenicity of casein and β-LG, IgE reactivity and basophil activation than PHWF1.
These results suggest that the tolerability of PHF1 and PHF2 in patients with CMA is similar to and higher than that of PHWF1, respectively, and that the degree of IgE binding to PHFs does not necessarily correspond to basophil activation.
当无法进行纯母乳喂养时，部分水解配方奶粉（PHF）常被用作婴儿的起始配方奶粉。一些儿童在首次摄入牛乳蛋白后会出现过敏症状，包括过敏反应。因此，PHF在牛奶蛋白过敏（CMA）婴儿中的耐受性是重要信息。部分水解乳清配方奶粉（PHWF）已得到充分表征，但同时含有乳清和酪蛋白的配方奶粉也有。我们评估了两种乳清和酪蛋白PHF（PHF1和PHF2）的特性，并将它们与一种PHWF（PHWF1）进行比较。
使用酶联免疫吸附测定（ELISA）测量配方奶粉中β-乳球蛋白（β-LG）和酪蛋白的残留抗原性。使用高压液相色谱法测定分子量分布。分别通过免疫捕获抑制试验和使用CMA患者血液进行的嗜碱性粒细胞活化试验评估配方奶粉的IgE反应性和致敏活性。
所有参与者（n = 10）均有酪蛋白特异性IgE。PHF1中β-LG的抗原性与PHWF1中的相似，但酪蛋白的抗原性略高于PHWF1。PHF1的IgE反应性高于PHWF1。然而，PHF1和PHWF1激活嗜碱性粒细胞的能力相似。PHF2的酪蛋白和β-LG抗原性、IgE反应性和嗜碱性粒细胞活化均低于PHWF1。
这些结果表明，PHF1和PHF2在CMA患者中的耐受性分别与PHWF1相似且高于PHWF1，并且IgE与PHF结合的程度不一定与嗜碱性粒细胞活化相对应。
