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内皮细胞模拟表面修饰改善抗血栓形成能力并增强大鼠和猪血管移植物的通畅性。

Endothelium-Mimetic Surface Modification Improves Antithrombogenicity and Enhances Patency of Vascular Grafts in Rats and Pigs.

作者信息

Wei Yongzhen, Jiang Huan, Chai Chao, Liu Pei, Qian Meng, Sun Na, Gao Man, Zu Honglin, Yu Yongquan, Ji Guangbo, Zhang Yating, Yang Sen, He Ju, Cheng Jiansong, Tian Jinwei, Zhao Qiang

机构信息

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Tianjin, China.

State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials (Ministry of Education), Frontiers Science Center for Cell Responses, College of Life Sciences, Nankai University, Tianjin, China.

出版信息

JACC Basic Transl Sci. 2023 May 3;8(7):843-861. doi: 10.1016/j.jacbts.2022.12.009. eCollection 2023 Jul.

DOI:10.1016/j.jacbts.2022.12.009
PMID:37547067
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10401295/
Abstract

We first identified thrombomodulin (TM) and endothelial nitric oxide (NO) synthase as key factors for the antithrombogenic function of the endothelium in human atherosclerotic carotid arteries. Then, recombinant TM and an engineered galactosidase responsible for the conversion of an exogenous NO prodrug were immobilized on the surface of the vascular grafts. Surface modification by TM and NO cooperatively enhanced the antithrombogenicity and patency of vascular grafts. Importantly, we found that the combination of TM and NO also promoted endothelialization, whereas it reduced adverse intimal hyperplasia, which is critical for the maintenance of vascular homeostasis, as confirmed in rat and pig models.

摘要

我们首先确定血栓调节蛋白（TM）和内皮型一氧化氮（NO）合酶是人类动脉粥样硬化颈动脉内皮抗血栓形成功能的关键因素。然后，将重组TM和一种负责转化外源性NO前药的工程化半乳糖苷酶固定在血管移植物表面。TM和NO协同进行的表面修饰增强了血管移植物的抗血栓形成能力和通畅性。重要的是，我们发现TM和NO的组合还促进了内皮化，同时减少了内膜增生，这对维持血管稳态至关重要，大鼠和猪模型实验证实了这一点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16ee/10401295/2f2a880ee1de/gr8.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16ee/10401295/783fa4dc98c5/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16ee/10401295/78e05503a104/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16ee/10401295/b6748543a714/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16ee/10401295/24398266ed2a/gr3.jpg
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