Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
Microb Pathog. 2023 Oct;183:106279. doi: 10.1016/j.micpath.2023.106279. Epub 2023 Aug 5.
Clostridium perfringens, a common foodborne pathogen, exhibit high-stress resistance. The prevailing reliance on antibiotics in the farming industry for its prevention and control has led to increasing concerns over antibiotic residue and bacterial resistance. Bacteriophages that possess specific lytic activity against C. perfringens are of significant interest. Here, a novel C. perfringens phage, named vB_CP_qdyz_P5, was isolated and characterized. The phage displayed high stability at temperatures below 70 °C and pH levels ranging from 4 to 12. Genome analysis revealed that vB_CP_qdyz_P5 has a double-stand DNA of 18,888 bp with a G + C composition of 28.8%. Among the 27 identified opening reading frames (ORFs), eight were found to be functional genes. BLASTn analysis showed that vB_CP_qdyz_P5 is closely related to phage DCp1, with a genome homology coverage of 83%. Phylogenetic analysis indicated that vB_CP_qdyz_P5 may be a novel phage of the family Guelinviridae, Susfortunavirus. This study provides important preliminary information for further research on the potential use of vB_CP_qdyz_P5 in protecting against C. perfringens and maintaining intestinal health.
产气荚膜梭菌是一种常见的食源性病原体，具有较强的抗压能力。由于农业生产中普遍依赖抗生素来预防和控制该病原体，因此人们越来越关注抗生素残留和细菌耐药性问题。能够特异性裂解产气荚膜梭菌的噬菌体备受关注。本研究分离并鉴定了一株新型产气荚膜梭菌噬菌体，命名为 vB_CP_qdyz_P5。该噬菌体在低于 70°C 的温度和 pH4-12 的范围内具有较高的稳定性。基因组分析表明，vB_CP_qdyz_P5 的双链 DNA 全长为 18888bp，GC 含量为 28.8%。在鉴定出的 27 个开放阅读框（ORFs）中，有 8 个被预测为功能基因。BLASTn 分析表明，vB_CP_qdyz_P5 与噬菌体 DCp1 具有高度同源性，基因组同源性覆盖率为 83%。系统发育分析表明，vB_CP_qdyz_P5 可能是一种新型的 Guelevirus 科、Susfortunavirus 噬菌体。本研究为进一步研究 vB_CP_qdyz_P5 预防产气荚膜梭菌感染和维持肠道健康的潜在应用提供了重要的初步信息。
