Fukushima M, Kobayashi T
J Appl Physiol (1985). 1986 Jun;60(6):1828-33. doi: 10.1152/jappl.1986.60.6.1828.
We tested the effects of OKY-046, a thromboxane synthase inhibitor, on lung injury induced by 2 h of pulmonary air infusion (1.23 ml/min) in the pulmonary artery of unanesthetized sheep with chronic lung lymph fistula so as to assess the role of thromboxane A2 (TxA2) in the lung injury. We measured pulmonary hemodynamic parameters and the lung fluid balance. The concentrations of thromboxane B2 (TxB2) and 6-ketoprostaglandin F1 alpha (6-keto-PGF1 alpha) in plasma and lung lymph were determined by radioimmunoassay. Air infusion caused sustained pulmonary hypertension and an increase in pulmonary vascular permeability. The levels of TxB2 and 6-keto-PGF1 alpha in both plasma and lung lymph were significantly elevated during the air infusion. TxB2 concentration in plasma obtained from the left atrium was higher than that from the pulmonary artery at 15 min of air infusion. When sheep were pretreated with OKY-046 (10 mg/kg iv) prior to the air infusion, increases in TxB2 were prevented. The pulmonary arterial pressure, however, increased similarly to that of untreated sheep (1.8 X base line). The increase in lung lymph flow was significantly suppressed during the air infusion. Our data suggest that the pulmonary hypertension observed during air embolism is not caused by TxA2.
我们测试了血栓素合酶抑制剂OKY - 046对慢性肺淋巴瘘未麻醉绵羊肺动脉内2小时肺充气（1.23毫升/分钟）所致肺损伤的影响，以评估血栓素A2（TxA2）在肺损伤中的作用。我们测量了肺血流动力学参数和肺液体平衡。通过放射免疫分析法测定血浆和肺淋巴中血栓素B2（TxB2）和6 - 酮前列腺素F1α（6 - 酮 - PGF1α）的浓度。肺充气导致持续性肺动脉高压和肺血管通透性增加。在肺充气期间，血浆和肺淋巴中TxB2和6 - 酮 - PGF1α的水平均显著升高。在肺充气15分钟时，从左心房获取的血浆中TxB2浓度高于肺动脉中的浓度。当绵羊在肺充气前用OKY - 046（10毫克/千克静脉注射）预处理时，TxB2的升高被阻止。然而，肺动脉压的升高与未治疗的绵羊相似（为基线的1.8倍）。在肺充气期间，肺淋巴流量的增加被显著抑制。我们的数据表明，空气栓塞期间观察到的肺动脉高压不是由TxA2引起的。
