Floros J, Steinbrink R, Jacobs K, Phelps D, Kriz R, Recny M, Sultzman L, Jones S, Taeusch H W, Frank H A
J Biol Chem. 1986 Jul 5;261(19):9029-33.
A group of 35,000-dalton sialoglycoproteins is the major non-serum protein component of pulmonary surfactant. Tryptic fragments of these proteins were sequenced, and oligonucleotide probes were synthesized based on the amino acid sequences. A human lung cDNA library was then screened using the oligonucleotide probes, and clones coding for these proteins were identified and characterized. By in vitro transcription-translation experiments we have associated individual clones with particular proteins. The data suggest that co-translational modifications of two primary translation products account for many of the isoforms observed by two-dimensional gel electrophoresis in the precursors of 35,000-dalton sialoglycoproteins.
一组35000道尔顿的唾液糖蛋白是肺表面活性剂的主要非血清蛋白成分。对这些蛋白质的胰蛋白酶片段进行了测序，并根据氨基酸序列合成了寡核苷酸探针。然后使用寡核苷酸探针筛选人肺cDNA文库，鉴定并表征了编码这些蛋白质的克隆。通过体外转录-翻译实验，我们将各个克隆与特定蛋白质联系起来。数据表明，两种初级翻译产物的共翻译修饰导致了在35000道尔顿唾液糖蛋白前体的二维凝胶电泳中观察到的许多同工型。
