Regulatory roles of lncRNA in breast cancer cell proliferation.

BACKGROUND

Breast cancer represents the second most deadly malignancy in women, and long noncoding RNAs (lncRNAs) have crucial functions in its development.

OBJECTIVE

To investigate effects of the promoter of antisense DNA damage-activated RNA () on epithelial-mesenchymal transition (EMT) in breast cancer cells and their proliferation.

METHODS

lncRNAs potentially regulating the transcriptional activity of the E-cadherin (E-cad, an epithelial cell marker) gene promoter were screened using a dual-luciferase reporter assay. was overexpressed in Michigan cancer foundation 7 (MCF-7) breast cancer cells. E-cad and N-cadherin (N-cad, a mesenchymal cell marker) levels were detected by immunoblotting. Cell viability was assessed using a cell counting kit-8.

RESULTS

and / conservatively regulated the promoter activity of . overexpression in MCF-7 inhibited E-cad expression, but upregulated N-cad. The enhanced expression of promoted cell proliferation.

CONCLUSION

is a key transcriptional repressor of and has regulatory effects on the promotion of cell proliferation. is an oncogene in breast cancer, potentially facilitating the EMT process and promoting cell proliferation.