[长链非编码RNA靶向作用对儿童急性淋巴细胞白血病细胞增殖和凋亡的影响]
[Effect of LncRNA Targeting on Cell Proliferation and Apoptosis in Childhood Acute Lymphoblastic Leukemia Cells].
作者信息
Qin Yan, Yun Xue-Xue, Zheng Zhong-Mei, Xu Qian, Zuo Li-Min
机构信息
Department of Emergency, The Affiliated Women's and Children's Hospital, School of Medicine, University of Electronic Science and Technology of China·Chengdu Women's and Children's Central Hospital, Chengdu 611731, Sichuan Province, China.
Department of Clinical Laboratory, The Affiliated Women's and Children's Hospital, School of Medicine, University of Electronic Science and Technology of China·Chengdu Women's and Children's Central Hospital, Chengdu 611731, Sichuan Province, China.
出版信息
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2023 Aug;31(4):1032-1037. doi: 10.19746/j.cnki.issn.1009-2137.2023.04.016.
OBJECTIVE
To investigate the effects of long non-coding RNA (lncRNA) antisense RNA 1 () targeting on the proliferation and apoptosis of childhood acute lymphoblastic leukemia (ALL) cells.
METHODS
RT-qPCR was used to determine the expression of and in the plasma of controls and ALL children. Human ALL cells Jurkat were divided into si-GATA3-AS1, si-NC, miR-NC, miR-515-5p, si-GATA3-AS1+anti-miR-NC and si-GATA3-AS1+anti-miR-515-5p groups. CCK-8 assay was used to detect the cell proliferation, and flow cytometry was used to detect the cell apoptosis. The targeting relationship between and was determined by dual-luciferase reporter assay.
RESULTS
The expression level of in the plasma of ALL children was significantly higher than that of controls ( <0.001), while the expression level of was significantly lower than that of controls ( <0.001). Compared with the si-NC group, the cell inhibition rate, apoptosis rate, and expression level in si-GATA3-AS1 group were significantly increased ( <0.001). Compared with the miR-NC group, the cell inhibition rate and apoptosis rate in miR-515-5p group were significantly increased ( <0.001). could directly and specifically bind to . Compared with the si-GATA3-AS1+anti-miR-NC group, the cell inhibition rate and apoptosis rate in si-GATA3-AS1+anti-miR-515-5p group were significantly decreased ( <0.001).
CONCLUSION
Down-regulation of can inhibit proliferation and induce apoptosis of childhood ALL cells by targeting up-regulation of expression.
目的
探讨靶向长链非编码RNA（lncRNA）反义RNA 1（）对儿童急性淋巴细胞白血病（ALL）细胞增殖和凋亡的影响。
方法
采用RT-qPCR检测对照组和ALL患儿血浆中及的表达。将人ALL细胞Jurkat分为si-GATA3-AS1组、si-NC组、miR-NC组、miR-515-5p组、si-GATA3-AS1+anti-miR-NC组和si-GATA3-AS1+anti-miR-515-5p组。采用CCK-8法检测细胞增殖，流式细胞术检测细胞凋亡。通过双荧光素酶报告基因检测法确定与的靶向关系。
结果
ALL患儿血浆中的表达水平显著高于对照组（<0.001），而的表达水平显著低于对照组（<0.001）。与si-NC组相比，si-GATA3-AS1组的细胞抑制率、凋亡率及表达水平显著升高（<0.00。与miR-NC组相比，miR-515-5p组的细胞抑制率和凋亡率显著升高（<0.001）。可直接特异性结合。与si-GATA3-AS1+anti-miR-NC组相比，si-GATA3-AS1+anti-miR-515-5p组的细胞抑制率和凋亡率显著降低（<0.001）。
结论
下调可通过靶向上调的表达抑制儿童ALL细胞的增殖并诱导其凋亡。
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