Zhou Nan, Cao Shu-Xing, Wang Zhen-Zhen, Luo Jian-Min, Liu Xiao-Jun, Shang Yin-Tao, Yang Lin
Department of Hematology,The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
Department of Orthopedics, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2023 Aug;31(4):1093-1099. doi: 10.19746/j.cnki.issn.1009-2137.2023.04.025.
To investigate the effect of resveratrol (RSV) on the proliferation of multiple myeloma (MM) cells and its molecular mechanism.
MM cells (MM1.S, RPMI-8226 and U266) were treated with different concentrations of RSV for 24-72 h. The effect of RSV on the proliferation of MM cells was detected by CCK-8 (cell counting kit-8) assay. RPMI-8226 cells were divided into RSV, miR-21 mimic, RSV+miR-21 mimic, miR-21 inhibitor and RSV+miR-21 inhibitor groups, and transfected with corresponding plasmids. The cell cycle distribution of each group was detected by flow cytometry with propidium iodide (PI) single staining. The cell apoptosis of each group was detected by AnnexinV-FITC/PE-PI double staining. The expression of miR-21 in MM cells treated with RSV and the expression of mRNA in each group were detected by qRT-PCR. The expression of KLF5 protein in each group was detected by Western blot.
RSV inhibited the proliferation and induced apoptosis of MM cells in a time- and dose-dependent manner. After the MM cells were treated with RSV, the number of cells in sub-G phase was increased, and that in G/M phase was decreased. Moreover, RSV significantly downregulated the expression of miR-21 in MM cells, and the inhibitory effect of miR-21 mimic on expression in MM cells was counteracted by RSV.
RSV may inhibit the proliferation and induce apoptosis of MM cells by inhibiting miR-21 and up-regulating expression.
探讨白藜芦醇（RSV）对多发性骨髓瘤（MM）细胞增殖的影响及其分子机制。
用不同浓度的RSV处理MM细胞（MM1.S、RPMI-8226和U266）24 - 72小时。采用CCK-8（细胞计数试剂盒-8）法检测RSV对MM细胞增殖的影响。将RPMI-8226细胞分为RSV组、miR-21模拟物组、RSV + miR-21模拟物组、miR-21抑制剂组和RSV + miR-21抑制剂组，并用相应质粒进行转染。采用碘化丙啶（PI）单染法通过流式细胞术检测各组细胞周期分布。采用AnnexinV-FITC/PE-PI双染法检测各组细胞凋亡情况。采用qRT-PCR检测RSV处理的MM细胞中miR-21的表达及各组mRNA的表达。采用蛋白质免疫印迹法检测各组KLF5蛋白的表达。
RSV以时间和剂量依赖性方式抑制MM细胞增殖并诱导其凋亡。RSV处理MM细胞后，亚G期细胞数量增加，G/M期细胞数量减少。此外，RSV显著下调MM细胞中miR-21的表达，且RSV可抵消miR-21模拟物对MM细胞表达的抑制作用。
RSV可能通过抑制miR-21并上调表达来抑制MM细胞增殖并诱导其凋亡。
