通过 LAMP-CRISPR/Cas12b 与热不稳定尿嘧啶-DNA 糖基化酶的结合，实现对 的快速可视化检测，从而消除携带污染。
Rapid visual detection of by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination.
机构信息
Jiangsu Key Laboratory of Marine Biological Resources and Environment, Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Jiangsu Ocean University, Lianyungang 222005, China.
Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang 222005, China.
出版信息
J Zhejiang Univ Sci B. 2023 Aug 15;24(8):749-754. doi: 10.1631/jzus.B2200705.
Abstract
is a major pathogen frequently found in seafood. Rapid and accurate detection of this pathogen is important for the control of bacterial foodborne diseases and to ensure food safety. In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.
摘要
是一种常见于海鲜的主要病原体。快速准确地检测这种病原体对于控制细菌性食源性疾病和确保食品安全非常重要。在本研究中，我们建立了一种一管式系统，该系统结合了尿嘧啶-DNA 糖基化酶（UDG）、环介导等温扩增（LAMP）和簇状规则间隔短回文重复序列（CRISPR）/CRISPR 相关蛋白 12b（Cas12b），用于检测海鲜中的 。该检测系统可以使用单个管有效地进行鉴定，避免了交叉污染的风险。
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