一种针对呼吸道合胞病毒的中和单克隆抗体，它能与融合蛋白的F1和F2组分结合。

A neutralizing monoclonal antibody to respiratory syncytial virus which binds to both F1 and F2 components of the fusion protein.

作者信息

Samson A C, Willcocks M M, Routledge E G, Morgan L A, Toms G L

出版信息

J Gen Virol. 1986 Jul;67 ( Pt 7):1479-83. doi: 10.1099/0022-1317-67-7-1479.

Abstract

A virus-neutralizing monoclonal antibody (1E3) specifically immunoprecipitated the 70000 mol. wt. (70K) fusion (F) protein from respiratory syncytial (RS) virus-infected HeLa cells. Western blotting analysis of polypeptides from such cells separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions revealed that 1E3 was peculiar in that it bound to both F1 (50K) and F2 (20K) components of the F protein. Antibody subsequently eluted from either the F1 or the F2 regions of immunoblots re-bound to both F1 and F2 regions of the SDS-PAGE blot. These results show that monoclonal antibody 1E3 reacts with an epitope which is found on both F1 and F2 subunits of RS virus fusion protein.

摘要

一种病毒中和单克隆抗体（1E3）能特异性地从呼吸道合胞病毒（RS）感染的HeLa细胞中免疫沉淀出分子量为70000道尔顿（70K）的融合（F）蛋白。在还原条件下，对经十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）分离的此类细胞的多肽进行蛋白质印迹分析表明，1E3的独特之处在于它能与F蛋白的F1（50K）和F2（20K）组分结合。随后从免疫印迹的F1或F2区域洗脱的抗体重新与SDS - PAGE印迹的F1和F2区域结合。这些结果表明，单克隆抗体1E3与呼吸道合胞病毒融合蛋白的F1和F2亚基上均存在的一个表位发生反应。