呼吸道合胞病毒包膜糖蛋白之一GP90的纯化与特性分析
Purification and characterization of GP90, one of the envelope glycoproteins of respiratory syncytial virus.
作者信息
Walsh E E, Schlesinger J J, Brandriss M W
出版信息
J Gen Virol. 1984 Apr;65 ( Pt 4):761-7. doi: 10.1099/0022-1317-65-4-761.
PMID:6707612
Abstract
The large glycoprotein, GP90, of respiratory syncytial virus (RSV) was purified by affinity chromatography using a monoclonal antibody. Hyperimmune rabbit antiserum directed specifically to the purified GP90 neutralized RSV to high titre but did not inhibit fusion of previously infected cells. 125I-labelled GP90 bound rapidly to HEp-2 cells in an unsaturable manner; binding was inhibited by the hyperimmune rabbit antiserum.
摘要
呼吸道合胞病毒（RSV）的大糖蛋白GP90通过使用单克隆抗体的亲和层析法进行纯化。特异性针对纯化的GP90的超免疫兔抗血清能高效中和RSV，但不抑制先前感染细胞的融合。125I标记的GP90以不饱和方式迅速与HEp-2细胞结合；这种结合被超免疫兔抗血清所抑制。
Zotero 插件