• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

呼吸道合胞病毒包膜糖蛋白之一GP90的纯化与特性分析

Purification and characterization of GP90, one of the envelope glycoproteins of respiratory syncytial virus.

作者信息

Walsh E E, Schlesinger J J, Brandriss M W

出版信息

J Gen Virol. 1984 Apr;65 ( Pt 4):761-7. doi: 10.1099/0022-1317-65-4-761.

DOI:10.1099/0022-1317-65-4-761
PMID:6707612
Abstract

The large glycoprotein, GP90, of respiratory syncytial virus (RSV) was purified by affinity chromatography using a monoclonal antibody. Hyperimmune rabbit antiserum directed specifically to the purified GP90 neutralized RSV to high titre but did not inhibit fusion of previously infected cells. 125I-labelled GP90 bound rapidly to HEp-2 cells in an unsaturable manner; binding was inhibited by the hyperimmune rabbit antiserum.

摘要

呼吸道合胞病毒（RSV）的大糖蛋白GP90通过使用单克隆抗体的亲和层析法进行纯化。特异性针对纯化的GP90的超免疫兔抗血清能高效中和RSV，但不抑制先前感染细胞的融合。125I标记的GP90以不饱和方式迅速与HEp-2细胞结合；这种结合被超免疫兔抗血清所抑制。

相似文献

1
Purification and characterization of GP90, one of the envelope glycoproteins of respiratory syncytial virus.呼吸道合胞病毒包膜糖蛋白之一GP90的纯化与特性分析
J Gen Virol. 1984 Apr;65 ( Pt 4):761-7. doi: 10.1099/0022-1317-65-4-761.
2
Purification and characterization of the respiratory syncytial virus fusion protein.呼吸道合胞病毒融合蛋白的纯化与特性分析
J Gen Virol. 1985 Mar;66 ( Pt 3):409-15. doi: 10.1099/0022-1317-66-3-409.
3
Monoclonal antibodies to the large glycoproteins of respiratory syncytial virus: possible evidence for several functional antigenic sites.针对呼吸道合胞病毒大糖蛋白的单克隆抗体：多个功能性抗原位点的可能证据。
J Gen Virol. 1987 Aug;68 ( Pt 8):2161-7. doi: 10.1099/0022-1317-68-8-2161.
4
Immunological differences between the envelope glycoproteins of two strains of human respiratory syncytial virus.两株人呼吸道合胞病毒包膜糖蛋白之间的免疫学差异
J Gen Virol. 1987 Aug;68 ( Pt 8):2169-76. doi: 10.1099/0022-1317-68-8-2169.
5
Analysis of the respiratory syncytial virus fusion protein using monoclonal and polyclonal antibodies.使用单克隆抗体和多克隆抗体对呼吸道合胞病毒融合蛋白进行分析。
J Gen Virol. 1986 Mar;67 ( Pt 3):505-13. doi: 10.1099/0022-1317-67-3-505.
6
Evidence for the role of glycoprotein G of respiratory syncytial virus in binding of Neisseria meningitidis to HEp-2 cells.呼吸道合胞病毒糖蛋白G在脑膜炎奈瑟菌与HEp-2细胞结合中作用的证据。
FEMS Immunol Med Microbiol. 1994 Nov;10(1):25-30. doi: 10.1111/j.1574-695X.1994.tb00007.x.
7
A neutralizing monoclonal antibody to respiratory syncytial virus which binds to both F1 and F2 components of the fusion protein.一种针对呼吸道合胞病毒的中和单克隆抗体，它能与融合蛋白的F1和F2组分结合。
J Gen Virol. 1986 Jul;67 ( Pt 7):1479-83. doi: 10.1099/0022-1317-67-7-1479.
8
The purification of four respiratory syncytial virus proteins and their evaluation as protective agents against experimental infection in BALB/c mice.四种呼吸道合胞病毒蛋白的纯化及其作为抗BALB/c小鼠实验性感染保护剂的评估。
J Gen Virol. 1988 Feb;69 ( Pt 2):293-303. doi: 10.1099/0022-1317-69-2-293.
9
Human monoclonal antibodies isolated from spontaneous Epstein-Barr virus-transformed tumors of Hu-SPL-SCID mice and specific for fusion protein display broad neutralizing activity toward respiratory syncytial virus.从Hu-SPL-SCID小鼠的自发性爱泼斯坦-巴尔病毒转化肿瘤中分离出的、对融合蛋白具有特异性的人单克隆抗体，对呼吸道合胞病毒显示出广泛的中和活性。
J Infect Dis. 1999 Aug;180(2):268-77. doi: 10.1086/314876.
10
Glycoproteins of human parainfluenza virus type 3: affinity purification, antigenic characterization and reconstitution into lipid vesicles.人副流感病毒3型糖蛋白：亲和纯化、抗原特性鉴定及脂质体重构
J Gen Virol. 1987 Feb;68 ( Pt 2):409-18. doi: 10.1099/0022-1317-68-2-409.

引用本文的文献

1
Biophysical and flavonoid-binding studies of the G protein ectodomain of group A human respiratory syncytial virus.A组人呼吸道合胞病毒G蛋白胞外域的生物物理及类黄酮结合研究
Heliyon. 2019 Mar 25;5(3):e01394. doi: 10.1016/j.heliyon.2019.e01394. eCollection 2019 Mar.
2
Virus-Specific Antibody, Viral Load, and Disease Severity in Respiratory Syncytial Virus Infection.呼吸道合胞病毒感染中的病毒特异性抗体、病毒载量和疾病严重程度。
J Infect Dis. 2018 Jun 20;218(2):208-217. doi: 10.1093/infdis/jiy106.
3
Respiratory syncytial virus infection: from biology to therapy: a perspective.
呼吸道合胞病毒感染：从生物学到治疗：一个视角。
World Allergy Organ J. 2008 Feb;1(2):21-8. doi: 10.1097/WOX.0b013e31816549a2.
4
Diagnosis of respiratory syncytial virus infection: comparison of reverse transcription-PCR to viral culture and serology in adults with respiratory illness.呼吸道合胞病毒感染的诊断：成人呼吸道疾病中逆转录-聚合酶链反应与病毒培养及血清学检测的比较
J Clin Microbiol. 2002 Mar;40(3):817-20. doi: 10.1128/JCM.40.3.817-820.2002.
5
Respiratory syncytial virus infection: immune response, immunopathogenesis, and treatment.呼吸道合胞病毒感染：免疫反应、免疫发病机制及治疗
Clin Microbiol Rev. 1999 Apr;12(2):298-309. doi: 10.1128/CMR.12.2.298.
6
Heparin-like structures on respiratory syncytial virus are involved in its infectivity in vitro.呼吸道合胞病毒上的类肝素结构参与其体外感染性。
J Virol. 1998 Sep;72(9):7221-7. doi: 10.1128/JVI.72.9.7221-7227.1998.
7
Priming with secreted glycoprotein G of respiratory syncytial virus (RSV) augments interleukin-5 production and tissue eosinophilia after RSV challenge.用呼吸道合胞病毒（RSV）分泌的糖蛋白G进行预刺激可增强RSV攻击后白细胞介素-5的产生和组织嗜酸性粒细胞增多。
J Virol. 1998 Apr;72(4):2871-80. doi: 10.1128/JVI.72.4.2871-2880.1998.
8
Determination of the disulfide bond arrangement of human respiratory syncytial virus attachment (G) protein by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.利用基质辅助激光解吸/电离飞行时间质谱法测定人呼吸道合胞病毒附着（G）蛋白的二硫键排列
Protein Sci. 1997 Jun;6(6):1308-15. doi: 10.1002/pro.5560060619.
9
Generation of atypical pulmonary inflammatory responses in BALB/c mice after immunization with the native attachment (G) glycoprotein of respiratory syncytial virus.用呼吸道合胞病毒天然附着（G）糖蛋白免疫BALB/c小鼠后产生非典型肺部炎症反应。
J Virol. 1996 Nov;70(11):7783-91. doi: 10.1128/JVI.70.11.7783-7791.1996.
10
Isolation and characterization of a chimpanzee monoclonal antibody to the G glycoprotein of human respiratory syncytial virus.一种针对人呼吸道合胞病毒G糖蛋白的黑猩猩单克隆抗体的分离与鉴定。
Clin Diagn Lab Immunol. 1994 Nov;1(6):701-6. doi: 10.1128/cdli.1.6.701-706.1994.