McMahon K E, Farrell P M
J Nutr. 1986 Jun;116(6):936-43. doi: 10.1093/jn/116.6.936.
Lung surfactant and nonsurfactant phospholipid concentrations were analyzed in relation to choline status in male rats fed a choline-deficient (CD) or choline-supplemented (CS) diet over an 8-d period. On the first day plasma choline concentrations were significantly lower (11.5 +/- 0.9 micron) in rats fed the CD diet than in those fed the CS diet (16.1 +/- 1.2 microM). This relationship continued for the duration of the study. Hepatic phosphatidylcholine/phosphatidylethanolamine (PC/PE) ratios were significantly lower after d 1 of the CD diet, further decreased on d 2 (1.09 +/- 0.04) and remained low through d 8. Only on d 4 were lung surfactant PC and total phospholipid concentrations lower in rats fed the CD diet than in those fed the CS diet. The composition of surfactant, determined by the ratio of PC to total phospholipids, did not change. On both d 4 and d 8 the PC/PE ratios in the nonsurfactant fraction were lower in rats fed the CD diet than in those fed the CS diet. This shift in PC/PE ratio in the lung is similar to the PC/PE shift in the liver associated with dietary choline deficiency. The altered lung phospholipid concentrations in the nonsurfactant (residual) fraction on d 4 and d 8 suggest an adaptation in the lung's phospholipid metabolism to replenish the physiologically essential surfactant PC.
在为期8天的时间里，对喂食胆碱缺乏（CD）或胆碱补充（CS）饮食的雄性大鼠的肺表面活性物质和非表面活性物质磷脂浓度与胆碱状态的关系进行了分析。在第一天，喂食CD饮食的大鼠血浆胆碱浓度（11.5±0.9微摩尔）显著低于喂食CS饮食的大鼠（16.1±1.2微摩尔）。这种关系在研究期间一直持续。CD饮食第1天后，肝脏磷脂酰胆碱/磷脂酰乙醇胺（PC/PE）比值显著降低，在第2天进一步下降（1.09±0.04），并在第8天一直保持较低水平。仅在第4天，喂食CD饮食的大鼠肺表面活性物质PC和总磷脂浓度低于喂食CS饮食的大鼠。由PC与总磷脂的比值确定的表面活性物质组成没有变化。在第4天和第8天，喂食CD饮食的大鼠非表面活性物质部分的PC/PE比值均低于喂食CS饮食的大鼠。肺中PC/PE比值的这种变化类似于与饮食胆碱缺乏相关的肝脏中PC/PE的变化。第4天和第8天非表面活性物质（残余）部分肺磷脂浓度的改变表明肺磷脂代谢发生了适应性变化，以补充生理必需的表面活性物质PC。
