Department of Chemistry, Faculty of Art and Sciences, Balikesir University, Balikesir, Turkey.
J Mol Recognit. 2023 Sep;36(9):e3048. doi: 10.1002/jmr.3048. Epub 2023 Aug 8.
The aim of this study was to investigate the inhibitory effects of some pesticides known to have harmful effects on human health on carbonic anhydrase isoenzymes. Therefore, carbonic anhydrase isoenzymes (hCA I and II) were purified from human erythrocytes. The isoenzymes were purified from human erythrocytes by using an affinity column that has the chemical structure of Sepharose-4B-4-(6-amino-hexyloxy)-benzenesulfonamide. The purity of the isoenzymes was checked by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE). It was determined that the pesticides used in this study inhibit hCA I and hCA II isoenzymes at different levels in vitro. It was determined that the strongest inhibitor for the hCA I enzyme was Carbofuran (IC :6.52 μM; K : 3.58 μM) and the weakest one was 1-Naphtol (IC :16.55 μM; K : 14.4 μM) among these pesticides. It was also found that the strongest inhibitor for the hCA II enzyme was coumatetralil (IC :5.06 μM; K : 1.62 μM) and the weakest one was Dimethachlor (IC 14.6 μM; K : 8.44 μM).
本研究旨在探讨一些已知对人体健康有害的农药对碳酸酐酶同工酶的抑制作用。因此，从人红细胞中纯化了碳酸酐酶同工酶（hCA I 和 II）。同工酶通过具有 Sepharose-4B-4-（6-氨基己氧基）-苯磺酰胺化学结构的亲和柱从人红细胞中纯化。同工酶的纯度通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDSPAGE）进行检查。研究中使用的农药在体外以不同水平抑制 hCA I 和 hCA II 同工酶。结果表明，对 hCA I 酶抑制作用最强的抑制剂是呋喃丹（IC：6.52 μM；K：3.58 μM），最弱的抑制剂是 1-萘酚（IC：16.55 μM；K：14.4 μM）。还发现对 hCA II 酶抑制作用最强的抑制剂是氯鼠灵（IC：5.06 μM；K：1.62 μM），最弱的抑制剂是二甲戊乐灵（IC 14.6 μM；K：8.44 μM）。
