Dickson P W, Schreiber G
Neurosci Lett. 1986 May 23;66(3):311-5. doi: 10.1016/0304-3940(86)90037-6.
We have investigated the expression of the gene for transthyretin (prealbumin) in the human choroid plexus. RNA was isolated from the human choroid plexus, fractionated by electrophoresis in agarose gel and transferred onto a nitrocellulose filter membrane. Transthyretin messenger RNA (mRNA) was identified by hybridization to radioactive complementary DNA for rat transthyretin. The level of transthyretin mRNA in the human choroid plexus was found to be at least 40 times higher than in human liver, suggesting very active synthesis of transthyretin in the choroid plexus.
我们研究了转甲状腺素蛋白（前白蛋白）基因在人脉络丛中的表达。从人脉络丛中分离出RNA，通过琼脂糖凝胶电泳进行分级分离，然后转移到硝酸纤维素滤膜上。通过与大鼠转甲状腺素蛋白的放射性互补DNA杂交来鉴定转甲状腺素蛋白信使核糖核酸（mRNA）。发现人脉络丛中转甲状腺素蛋白mRNA的水平比人肝脏中至少高40倍，这表明脉络丛中转甲状腺素蛋白的合成非常活跃。
