Department of Dermatology, Shanghai Skin Disease Hospital, Shanghai, China.
Department of Dermatologic Surgery, Shanghai Skin Disease Hospital, Shanghai, China.
Biosci Biotechnol Biochem. 2023 Oct 25;87(11):1255-1264. doi: 10.1093/bbb/zbad104.
BMP9, a member of the TGF-β superfamily, reveals the great translational promise for it has been shown to have the strong effect of osteogenic activity in vitro and in vivo. However, the implantation of certain BMPs (bone morphogenetic proteins) into muscular tissues induces ectopic bone formation. BMPs induce osteoblastic differentiation in skeletal muscle, suggesting that myogenic stem cells, such as myoblasts, are the potential progenitors of osteoblasts during heterotopic bone differentiation. Here, we investigate the role of BMP9 during primary mouse myoblasts differentiation. We found BMP9 enhanced cell proliferation and reduced myogenic differentiation of primary mouse myoblasts. In addition, adenovirus-mediated overexpression of BMP9 delayed muscle regeneration after BaCl2-induced injury. ALK1 knockdown reversed the inhibition of myoblast differentiation induced by BMP9. Our data indicate that BMP9 inhibits myogenic differentiation in primary mouse myoblasts and delays skeletal muscle regeneration after injury.
骨形态发生蛋白 9（BMP9）是转化生长因子-β超家族的成员，它具有很强的成骨活性，在体外和体内都有很好的转化潜力。然而，某些 BMP （骨形态发生蛋白）植入肌肉组织会引起异位骨形成。BMP 诱导成骨细胞在骨骼肌中的分化，这表明肌源性干细胞，如成肌细胞，是异位骨分化过程中成骨细胞的潜在祖细胞。在这里，我们研究了 BMP9 在原代小鼠成肌细胞分化过程中的作用。我们发现 BMP9 增强了原代小鼠成肌细胞的增殖，减少了成肌分化。此外，腺病毒介导的 BMP9 过表达延迟了 BaCl2 诱导损伤后的肌肉再生。ALK1 敲低逆转了 BMP9 诱导的成肌细胞分化抑制。我们的数据表明，BMP9 抑制原代小鼠成肌细胞的成肌分化，并延迟损伤后的骨骼肌再生。
