Mercer J F, Grimes A
FEBS Lett. 1986 Jul 28;203(2):185-90. doi: 10.1016/0014-5793(86)80739-6.
A number of cDNA clones encoding human ceruloplasmin were identified using two mixed oligonucleotide probes. One of these clones was shown by DNA sequence analysis to span from the complete N-terminal leader sequence to 114 amino acids short of the C-terminus. The leader sequence consists of 19 primarily hydrophobic amino acids. Northern blot analysis of RNA from human liver showed two species of ceruloplasmin mRNA; a minor species of 3600 nucleotides and a major one of 4400 nucleotides.
使用两种混合寡核苷酸探针鉴定出了多个编码人铜蓝蛋白的cDNA克隆。通过DNA序列分析表明，其中一个克隆涵盖了完整的N端前导序列直至距C端差114个氨基酸处。前导序列由19个主要为疏水性的氨基酸组成。对来自人肝脏的RNA进行Northern印迹分析显示有两种铜蓝蛋白mRNA；一种是3600个核苷酸的次要种类，另一种是4400个核苷酸的主要种类。
