Tanaka Y, Takei T, Aiba T, Masuda K, Kiuchi A, Fujiwara T
J Lipid Res. 1986 May;27(5):475-85.
We have previously reported the development of a reconstituted lung surfactant consisting of an organic solvent extract of natural bovine lung surfactant supplemented with synthetic lipids. This "artificial" surfactant was used successfully to treat surfactant deficiency states both in animals and humans. We now report on the successful testing of a synthetic lung surfactant consisting of a lipid-bound protein isolated from natural lung surfactant and the lipids present in the "artificial" lung surfactant and now used in the same concentration but in a synthetic, commercially available form. The synthetic lung surfactant possessed the in vitro and in vivo surface properties characterizing the "artificial" lung surfactant. In order to identify the components of the synthetic lung surfactant that are responsible for the required surface properties, a series of 25 simple mixtures was prepared. Of these, three possessed surface properties very similar to those of the "artificial" lung surfactant and the synthetic lung surfactant, in vitro as well as in vivo. These three mixtures had four components in common. Besides dipalmitoyl phosphatidylcholine and the lipid-bound protein, they each had a saturated fatty acid, palmitic or stearic, and they each had an acidic phospholipid, phosphatidylglycerol or phosphatidylserine.
我们之前曾报道过一种重组肺表面活性剂的研发情况，该表面活性剂由天然牛肺表面活性剂的有机溶剂提取物补充合成脂质组成。这种“人工”表面活性剂已成功用于治疗动物和人类的表面活性剂缺乏症。我们现在报告一种合成肺表面活性剂的成功测试情况，该合成肺表面活性剂由从天然肺表面活性剂中分离出的脂质结合蛋白以及“人工”肺表面活性剂中存在的脂质组成，现在以相同浓度但合成的、市售的形式使用。这种合成肺表面活性剂具有表征“人工”肺表面活性剂的体外和体内表面特性。为了确定合成肺表面活性剂中负责所需表面特性的成分，制备了一系列25种简单混合物。其中三种混合物的表面特性在体外和体内都与“人工”肺表面活性剂和合成肺表面活性剂非常相似。这三种混合物有四个共同成分。除了二棕榈酰磷脂酰胆碱和脂质结合蛋白外，它们各自都有一种饱和脂肪酸，棕榈酸或硬脂酸，并且它们各自都有一种酸性磷脂，磷脂酰甘油或磷脂酰丝氨酸。
