Barkai G, Reichman B, Modan M, Meir E, Goldman B, Mashiach S
Obstet Gynecol. 1986 Aug;68(2):168-72.
High-speed centrifugation of amniotic fluid enables separation of surfactant-containing lamellar bodies. Fifty-three amniotic fluid samples from pregnancies of 28 to 39 weeks' gestation were examined for fetal lung maturity by measuring fluorescence polarization (P). For each sample the P value was determined at 37C on both the whole amniotic fluid and on the surfactant-containing lamellar bodies obtained by centrifugation at 10,000 X g. A close linear correlation (r = 0.80) was found between the P value of the amniotic fluid samples and that of the lamellar bodies. There was no difference in the specificity for discrimination of hyaline membrane disease cases by both methods (93.5%) with 95% confidence limits of 82.1 to 98.6%. Because high-speed centrifugation enables the selective sedimentation of pulmonary surfactant, determination of the lamellar body P value may offer a practical solution for the estimation of fetal lung maturity in amniotic fluid samples contaminated with blood.
羊水的高速离心能够分离出含表面活性剂的板层小体。通过测量荧光偏振（P）对53份妊娠28至39周孕妇的羊水样本进行胎儿肺成熟度检测。对于每个样本，在37℃下分别测定全羊水以及通过10000×g离心获得的含表面活性剂板层小体的P值。发现羊水样本的P值与板层小体的P值之间存在密切的线性相关性（r = 0.80）。两种方法对透明膜病病例的鉴别特异性无差异（93.5%），95%置信区间为82.1%至98.6%。由于高速离心能够使肺表面活性剂选择性沉降，因此测定板层小体P值可能为估计受血液污染的羊水样本中的胎儿肺成熟度提供一种实用的解决方案。
