Department of Genetics and Plant Breeding, S.V. Agricultural College, Acharya NG Ranga Agricultural University (ANGRAU), Tirupati, 517502, Andhra Pradesh, India.
Department of Statistics and Computer Applications, S.V. Agricultural College, Acharya NG Ranga Agricultural University (ANGRAU), Tirupati, 517502, Andhra Pradesh, India.
Mol Biol Rep. 2023 Oct;50(10):8177-8188. doi: 10.1007/s11033-023-08714-9. Epub 2023 Aug 9.
The eventual shifting of cultivation method from puddle transplanted rice to direct-seeded rice (DSR) to save water prompted researchers to develop DSR-suitable varieties. To achieve this, identification of molecular markers associated with must-have traits for DSR, especially early seedling vigour related traits is crucial.
In the present investigation, the haplotype analysis using flanking markers of three important quantitative trait loci (QTLs) for early seedling vigour-related traits viz., qSV-6a (RM204 and RM402) for root length; qVI (RM20429 and RM3) for seedling vigour index; qGP-6 (RM528 and RM400) for germination percentage revealed that the marker alleles were found to show significant associations with qVI and qGP-6 QTLs. The majority of genotypes with high early seedling vigour are with qVIHap-1 (220 and 160 bp) and qGPHap-1 (290 and 290 bp). The rice genotypes with superior haplotypes for early seedling vigour are BMF536, BMF540, BMF525, MM129 and MDP2.
In conclusion, here we demonstrated that the markers RM20429 and RM3 are associated with seedling vigour index whereas RM528 and RM400 are associated with germination percentage. Therefore, these markers can be utilized to develop varieties suitable for DSR conditions through haplotype-based breeding. In addition, the rice genotypes with superior haplotypes can be of immense value to use as donors or can be released as varieties also under DSR conditions.
为了节约水资源，水稻的种植方式最终从水育秧变为了直播，这促使研究人员开发出适合直播的品种。为了实现这一目标，鉴定与直播必需特性相关的分子标记，尤其是与早期幼苗活力相关的特性，至关重要。
在本研究中，利用三个与早期幼苗活力相关性状的重要数量性状位点（QTL）侧翼标记（qSV-6a 基因的 RM204 和 RM402 用于根长；qVI 基因的 RM20429 和 RM3 用于幼苗活力指数；qGP-6 基因的 RM528 和 RM400 用于发芽率）进行单倍型分析，结果表明，标记等位基因与 qVI 和 qGP-6 QTL 显著相关。具有高早期幼苗活力的大多数基因型都具有 qVIHap-1（220 和 160 bp）和 qGPHap-1（290 和 290 bp）。具有优异早期幼苗活力的水稻基因型有 BMF536、BMF540、BMF525、MM129 和 MDP2。
总之，我们证明了 RM20429 和 RM3 标记与幼苗活力指数相关，而 RM528 和 RM400 标记与发芽率相关。因此，这些标记可用于通过基于单倍型的育种来开发适合直播条件的品种。此外，具有优异单倍型的水稻基因型可以作为供体非常有价值，也可以在直播条件下作为品种释放。
