Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan; Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan; Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan; Center for iPS Cell Research and Application (CiRA), Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Curr Opin Genet Dev. 2023 Oct;82:102091. doi: 10.1016/j.gde.2023.102091. Epub 2023 Aug 7.
During fetal oocyte development in mammals, germ cells progress through meiotic prophase I to form primordial follicles with pregranulosa cells. The primordial follicles remain dormant until oogenesis resumes during puberty. Studies in mice have elucidated mechanisms governing oogenesis, leading to the successful induction of functional oocytes from mouse pluripotent stem cells in vitro. Based on the in vivo/in vitro knowledge in mice and the histological and transcriptomic evidence for fetal oocyte development in humans and primates, human/primate oocyte-like cells corresponding to the early stage of oocytes in vivo have been successfully induced in vitro. Here, we discuss recent advances in our understanding of the mechanisms of fetal oocyte development in mammals, as well as in in vitro oogenesis.
在哺乳动物的胎儿卵母细胞发育过程中，生殖细胞经历减数分裂前期 I 以形成具有前颗粒细胞的原始卵泡。原始卵泡一直处于休眠状态，直到青春期恢复卵母细胞发生。在小鼠中的研究阐明了控制卵母细胞发生的机制，导致从小鼠多能干细胞中成功体外诱导功能性卵母细胞。基于小鼠体内/体外的知识以及人类和灵长类动物胎儿卵母细胞发育的组织学和转录组学证据，已经成功地在体外诱导出与体内早期卵母细胞相对应的人类/灵长类卵母细胞样细胞。在这里，我们讨论了我们对哺乳动物胎儿卵母细胞发育以及体外卵母细胞发生机制的理解的最新进展。
