Kuzara D, Zoltan B J, Greathouse S L, Jordan C W, Kohler C A
J Pharmacol Methods. 1986 Aug;16(1):73-82. doi: 10.1016/0160-5402(86)90030-6.
Commercially available instrumentation for conducting platelet aggregation studies in clinical and research laboratories consists of one-, two-, or four-channel aggregometers used in conjunction with strip chart recorders. These instruments have limited utility in large-scale drug screening and evaluation of the mode of action of drugs or in the clinical diagnosis of platelet disorders. A new instrument, a computerized multichannel aggregometer system (CMPAS) has been developed to collect, display, and analyze platelet aggregation data. The system is comprised of a 24-channel Born-type aggregometer, interfaced to a Rockwell AIM-65 microcomputer through an analogue-to-digital converter and an Epson dot-matrix printer. Each channel is individually calibrated, and aggregation data can be collected on up to 24 different platelet-rich plasma samples simultaneously. Conversational programs written in BASIC prompt the user for the addition of agonists and inhibitors. The tracings for each channel are displayed simultaneously, and a program automatically analyzes the data to generate the following parameters: baseline optical density, maximum aggregation response, positive and negative slopes, time to peak aggregation, and percentage response. Computerized multichannel aggregometer system data outputs are comparable to data generated by a standard Chronolog aggregometer unit. The advantages of the system include multichannel capability, simultaneous display of all channels allowing relative comparisons between control and experimental groups, and time savings and improved efficiency in conducting and analyzing aggregation experiments.
临床和研究实验室中用于进行血小板聚集研究的市售仪器包括与条形图记录仪结合使用的单通道、双通道或四通道血小板聚集仪。这些仪器在大规模药物筛选和药物作用方式评估或血小板疾病的临床诊断中的效用有限。一种新的仪器，即计算机化多通道血小板聚集仪系统（CMPAS）已被开发出来，用于收集、显示和分析血小板聚集数据。该系统由一台24通道的博恩型血小板聚集仪组成，通过模数转换器和爱普生点阵打印机与罗克韦尔AIM - 65微型计算机相连。每个通道都单独校准，并且可以同时收集多达24个不同富血小板血浆样本的聚集数据。用BASIC编写的对话程序提示用户添加激动剂和抑制剂。每个通道的描记图同时显示，并且一个程序会自动分析数据以生成以下参数：基线光密度、最大聚集反应、正负斜率、聚集峰值时间和反应百分比。计算机化多通道血小板聚集仪系统的数据输出与标准Chronolog血小板聚集仪单元生成的数据相当。该系统的优点包括多通道功能、所有通道的同时显示允许对照组和实验组之间进行相对比较，以及在进行和分析聚集实验时节省时间和提高效率。
