College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.
Sichuan Friendship Hospital, Chengdu, China.
J Immunol Methods. 2023 Sep;520:113534. doi: 10.1016/j.jim.2023.113534. Epub 2023 Aug 7.
This study aimed to establish a highly sensitive time-resolved fluorescence immunoassay of growth stimulating express gene 2 protein (ST2-TRFIA) and evaluate its application value for sepsis.
Two types of ST2 monoclonal specific antibodies against different epitopes of antigen molecule were used as coating and Eu-labeled antibodies. The double-antibody sandwich method was used in establishing ST2-TRFIA, and the methodology was evaluated. The established ST2-TRFIA was used in detecting ST2 concentration in the plasma samples of healthy controls and sepsis.
The linear range of ST2-TRFIA was 1.446-500 ng/mL. Plasma ST2 concentrations detected through ST2-TRFIA were consistent with the results of fluorescence quantitative immunochromatography (ρ = 0.946). The plasma ST2 concentrations of patients with sepsis were significantly higher than those of healthy controls (P < 0.01).
This study successfully established a highly sensitive ST2-TRFIA, which was highly comparable to commercially available fluorescent quantitative immunochromatographic kits and can facilitate the timely diagnosis of sepsis.
本研究旨在建立一种高灵敏度的生长刺激表达基因 2 蛋白（ST2-TRFIA）时间分辨荧光免疫分析方法，并评估其在脓毒症中的应用价值。
使用针对抗原分子不同表位的两种类型的 ST2 单克隆特异性抗体作为包被和 Eu 标记抗体。建立 ST2-TRFIA 时采用双抗体夹心法，并对该方法进行评估。用建立的 ST2-TRFIA 检测健康对照组和脓毒症患者的血浆样本中的 ST2 浓度。
ST2-TRFIA 的线性范围为 1.446-500ng/mL。通过 ST2-TRFIA 检测的血浆 ST2 浓度与荧光定量免疫层析法的结果一致（ρ=0.946）。脓毒症患者的血浆 ST2 浓度明显高于健康对照组（P<0.01）。
本研究成功建立了一种高灵敏度的 ST2-TRFIA，与市售荧光定量免疫层析试剂盒高度可比，可有助于脓毒症的及时诊断。
