Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt, Germany.
Department of Pharmacy, Ludwig-Maximilians-Universität München, Munich, Germany.
Methods Mol Biol. 2023;2706:125-135. doi: 10.1007/978-1-0716-3397-7_9.
Reporter gene assays are critical tools of nuclear receptor research for characterizing the effects of ligands on nuclear receptor activity. Common luciferase-based techniques require expensive substrates and are typically performed in endpoint format. Here, we describe a versatile reporter gene assay to observe nuclear receptor activity with fluorescent proteins as reporters. This setting is highly cost-efficient and enables observation of nuclear receptor activity over time with multiple measurements from one plate.
报告基因检测是核受体研究的关键工具，用于描述配体对核受体活性的影响。常见的基于荧光素酶的技术需要昂贵的底物，并且通常以终点格式进行。在这里，我们描述了一种灵活的报告基因检测方法，该方法使用荧光蛋白作为报告基因来观察核受体活性。这种设置具有很高的成本效益，并且能够在一个平板上进行多次测量，从而实时观察核受体活性。
