Department of Ophthalmology, Stanford University School of Medicine, Palo Alto, CA, USA.
Methods Mol Biol. 2023;2708:57-69. doi: 10.1007/978-1-0716-3409-7_7.
In this chapter, we describe a clinically relevant inducible and reversible ocular hypertension glaucoma model, which mimics the secondary glaucoma that can be a postoperative complication when silicone oil (SO) is used as a tamponade agent in human vitreoretinal surgery. First, we detail the procedures for generating SO-induced ocular hypertension (SOHU) in mouse and describe the two variations of this model that simulate common but distinct glaucoma types. We also describe separately the related procedures for measuring IOP and removing SO to return IOP to normal. Lastly, we describe the extension of the SOHU model in nonhuman primate (NHP), which recapitulates the severe neurodegeneration of acute human glaucoma but with unique dynamic changes of IOP due to the tolerance of the NHP ciliary body. The SOHU glaucoma model is, therefore, suitable for assessing experimental therapies for neuroprotection and regeneration, with or without treatment to lower IOP (SO removal), and consequently for translating relevant findings into novel and effective clinical treatments for glaucoma and other neurodegenerations. This model is straightforward, does not require special equipment or repetitive procedures, closely simulates clinical situations, and may be applicable to diverse animal species although minor modifications may be required.
在本章中，我们描述了一种临床上相关的诱导性和可逆性眼高压青光眼模型，该模型模拟了硅油（SO）作为眼内填充物在人眼玻璃体视网膜手术后引起的继发性青光眼，这是一种术后并发症。首先，我们详细介绍了在小鼠中产生 SO 诱导性眼高压（SOHU）的过程，并描述了该模型的两种变体，它们模拟了常见但不同类型的青光眼。我们还分别描述了测量眼压和去除 SO 以使眼压恢复正常的相关程序。最后，我们描述了在非人灵长类动物（NHP）中扩展的 SOHU 模型，该模型重现了急性人类青光眼的严重神经退行性变，但由于 NHP 睫状体的耐受性，眼压具有独特的动态变化。因此，SOHU 青光眼模型适合评估神经保护和再生的实验疗法，无论是否进行降低眼压（去除 SO）的治疗，从而将相关发现转化为治疗青光眼和其他神经退行性疾病的新颖有效的临床治疗方法。该模型简单易行，不需要特殊设备或重复程序，能紧密模拟临床情况，并且可能适用于多种动物物种，尽管可能需要进行一些微小的修改。
