TRPA1通道在HEK293细胞表达系统中对有害化学物质的辨别
Noxious chemical discrimination by TRPA1 channel in the HEK293 cell expression system.
作者信息
Shimomura Kenji, Oikawa Hinoki, Yamamoto Kosuke, Terajima Takehito, Yajima Shunsuke, Tomizawa Motohiro
机构信息
Department of Chemistry for Life Sciences and Agriculture, Tokyo University of Agriculture, Tokyo, 156-8502, Japan.
Department of Bioscience, Tokyo University of Agriculture, Tokyo, 156-8502, Japan.
出版信息
Curr Res Insect Sci. 2023 Jul 25;4:100066. doi: 10.1016/j.cris.2023.100066. eCollection 2023.
Nociception is the sensory perception of noxious chemical stimuli. Repellent behavior to avoid noxious stimuli is indispensable for survival, and this mechanism has been evolutionarily conserved across a wide range of species, from mammals to insects. The transient receptor potential ankyrin 1 (TRPA1) channel is one of the most conserved noxious chemical sensors. Here, we describe the heterologous stable expression of TRPA1 (TcTRPA1) in human embryonic kidney (HEK293) cells. The intracellular Ca influx was measured when two compounds, citronellal and -menthol, derived from plant essential oils, were applied using a fluorescence assay. The analysis revealed that citronellal evoked Ca influx dose-dependently for TcTRPA1, whereas -menthol did not. In combination with our present and previous results of the avoidance-behavioral assay at the organism level, we suggest that TcTRPA1 discriminates between these two toxic compounds, and diversification in the chemical nociception selectivity has occurred in TRPA1 channel among insect taxa.
伤害感受是对有害化学刺激的感觉认知。避免有害刺激的驱避行为对于生存至关重要，并且这种机制在从哺乳动物到昆虫的广泛物种中都通过进化得以保留。瞬时受体电位锚蛋白1（TRPA1）通道是最保守的有害化学传感器之一。在此，我们描述了TRPA1（TcTRPA1）在人胚肾（HEK293）细胞中的异源稳定表达。当使用荧光测定法施加两种源自植物精油的化合物香茅醛和薄荷醇时，测量细胞内的钙流入。分析表明，香茅醛对TcTRPA1剂量依赖性地诱发钙流入，而薄荷醇则不然。结合我们目前和之前在生物体水平上的回避行为测定结果，我们认为TcTRPA1能够区分这两种有毒化合物，并且在昆虫类群中TRPA1通道的化学伤害感受选择性已经发生了多样化。
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