代谢组学和蛋白质组学分析的结合为深入了解……生长差异背后的机制提供了线索。

Combination of metabolome and proteome analyses provides insights into the mechanism underlying growth differences in .

作者信息

Wu Xiaoyun, Lai Jiansheng, Chen Yeyu, Liu Ya, Song Mingjiang, Li Feiyang, Li Pengcheng, Li Qingzhi, Gong Quan

机构信息

The Fishery Institute of the Sichuan Academy of Agricultural Sciences, Chengdu 611730, China.

Fish Resources and Environment in the Upper Reaches of the Yangtze River Observation and Research Station of Sichuan Province, Chengdu 611730, China.

出版信息

iScience. 2023 Jul 18;26(8):107413. doi: 10.1016/j.isci.2023.107413. eCollection 2023 Aug 18.

To analyze the differences between different-sized , we randomly selected 600 3-month-old . juveniles. Four months later, the blood and white muscle of these fish were analyzed. The results showed no significant difference in the length-weight relationship (LWR) value between the large and small . . The levels of serum growth hormone (gh) and insulin-like growth factor 1 (igf1) in the large . were significantly lower than those in the small, whereas the activity levels of Total superoxide dismutase (T-sod) and catalase (cat) were opposite to the results of gh and igf1. A total of 212 and 245 metabolites showed significant changes in the positive and negative polarity mode, respectively. Among 3,308 proteins identified, 69 proteins showed upregulated expression, and 185 proteins showed downregulated expression. These results indicated that the growth advantage of . was closely related to glycolysis, protein synthesis, and antioxidant function.

为分析不同大小[具体对象未明确]之间的差异，我们随机选取了600条3月龄的[具体对象未明确]幼鱼。四个月后，对这些鱼的血液和白色肌肉进行了分析。结果表明，大、小[具体对象未明确]的体长-体重关系（LWR）值无显著差异。大鱼血清生长激素（gh）和胰岛素样生长因子1（igf1）水平显著低于小鱼，而总超氧化物歧化酶（T-sod）和过氧化氢酶（cat）的活性水平与gh和igf1的结果相反。在正、负极性模式下，分别有212种和245种代谢物显示出显著变化。在鉴定出的3308种蛋白质中，69种蛋白质表达上调，185种蛋白质表达下调。这些结果表明，[具体对象未明确]的生长优势与糖酵解、蛋白质合成和抗氧化功能密切相关。