使用 PENIA 检测血浆游离 κ 和 λ 轻链及其比值的参考区间。

Reference Intervals for Plasma Free Kappa and Lambda Chains and Kappa/Lambda Ratio using PENIA.

出版信息

Clin Lab. 2023 Aug 1;69(8). doi: 10.7754/Clin.Lab.2023.230109.

DOI:10.7754/Clin.Lab.2023.230109

Abstract

BACKGROUND

Determination of free light chains is used increasingly for patients with multiple myeloma and other plasma cell dyscrasias. The aim of the present study was to define reference intervals for free kappa and lambda chains and kappa/lambda ratio in lithium heparin plasma and compare the test results with cystatin C based estimated glomerular filtration rate.

METHODS

Free kappa and lambda chains were measured in Liheparin plasma from 222 healthy blood donors using free light chain reagents and a BNII nephelometer from Siemens Diagnostics.

RESULTS

Calculated reference intervals for kappa chain, free was 4.73 mg/L (90% confidence interval 4.00 - 5.45) - 22.66 mg/L (20.33 - 24.98), for lambda chain, free was 4.33 mg/L (3.70 - 4.95) - 29.28 mg/L (26.96 - 31.59), and for Kappa/Lambda Chain ratio 0.59 (0.56 - 0.62) - 1.46 (1.37 - 1.56). There was no need for gender or age specific reference intervals. Cystatin C based estimated glomerular filtration rate had a significant effect on the levels of free light chains.

CONCLUSIONS

The study presents reference intervals for plasma free light chains and compared results of estimated glomerular filtration rate with free light chains of kappa and lambda.

摘要

背景

游离轻链的测定越来越多地用于多发性骨髓瘤和其他浆细胞异常患者。本研究的目的是确定锂肝素血浆中游离κ和λ链以及κ/λ比值的参考区间，并将检测结果与基于胱抑素 C 的估计肾小球滤过率进行比较。

方法

使用游离轻链试剂和西门子诊断公司的 BNII 散射浊度仪，在 222 名健康献血者的 Liheparin 血浆中测量游离κ和λ链。

结果

计算得出游离κ链的参考区间为 4.73mg/L（90%置信区间 4.00-5.45）-22.66mg/L（20.33-24.98），游离λ链的参考区间为 4.33mg/L（3.70-4.95）-29.28mg/L（26.96-31.59），κ/λ 链比值为 0.59（0.56-0.62）-1.46（1.37-1.56）。无需性别或年龄特异性参考区间。基于胱抑素 C 的估计肾小球滤过率对游离轻链水平有显著影响。