Harigaya T, Nakayama K, Ohkubo H, Nakanishi S, Seo H, Hoshino K
Biochim Biophys Acta. 1986 Oct 16;868(1):30-8. doi: 10.1016/0167-4781(86)90083-7.
The present study was undertaken to find out whether or not sexual dimorphism in biological activities and amino acid compositions of mouse prolactin might be due to heterogeneity in mRNA for mouse prolactin Cloned cDNAs for mouse prolactin were first isolated from a mouse pituitary cDNA library by hybridization with a rat prolactin cDNA. Then, one clone of about 140 positive clones obtained from 2000 transformants was subjected to nucleotide sequence analysis and verified to contain a nearly full length of cDNA sequence coding for mouse prolactin precursor. The deduced complete amino acid sequence indicates that the precursor molecule consists of 31 amino acids as the signal peptide and 197 amino acids of prolactin, in which two amino acids were found to be different from the amino acid sequence previously published elsewhere. S1 nuclease mapping analysis using male and female pituitary RNAs indicates that mouse preprolactin is encoded by two mRNAs in both sexes. The two mRNAs differ from each other based upon the deletion of three nucleotides in the coding region for the signal peptide determined by the nucleotide sequence analysis in other cDNA clones. In the present study, no sexual difference was revealed in murine prolactin mRNA.
本研究旨在探究小鼠催乳素生物活性和氨基酸组成的性别二态性是否可能归因于小鼠催乳素mRNA的异质性。首先通过与大鼠催乳素cDNA杂交，从小鼠垂体cDNA文库中分离克隆小鼠催乳素的cDNA。然后，从2000个转化子中获得的约140个阳性克隆中的一个进行核苷酸序列分析，并验证其包含编码小鼠催乳素前体的近乎全长的cDNA序列。推导的完整氨基酸序列表明，前体分子由31个氨基酸的信号肽和197个氨基酸的催乳素组成，其中发现有两个氨基酸与其他地方先前发表的氨基酸序列不同。使用雄性和雌性垂体RNA进行的S1核酸酶图谱分析表明，小鼠催乳素原在两性中均由两种mRNA编码。根据其他cDNA克隆中的核苷酸序列分析，这两种mRNA在信号肽编码区域缺失三个核苷酸，彼此不同。在本研究中，未发现小鼠催乳素mRNA存在性别差异。
