Dept. Morphology and Physiology, Faculdade de Medicina do ABC, Centro Universitario FMABC, Santo Andre, SP, Brazil.
Dept. Physiology and Pathology, School of Dentistry, São Paulo State University - UNESP, Araraquara, SP, Brazil.
Brain Res. 2023 Nov 1;1818:148520. doi: 10.1016/j.brainres.2023.148520. Epub 2023 Aug 9.
The shell Nucleus Accumbens (NAcc) projects to the lateral preoptic area, which is involved in the central micturition control and receives inputs from medullary areas involved in cardiovascular control. We investigated the role of GABAergic and glutamatergic transmission in the shell NAcc on intravesical pressure (IP) and cardiovascular control. Male Wistar rats with guide cannulas implanted bilaterally in the shell NAcc 7 days prior to the experiments were anesthetized with 2% isoflurane in 100% O and subjected to cannulation of the femoral artery and vein for mean arterial pressure (MAP) and heart rate recordings (HR) and infusion of drugs, respectively. The urinary bladder (UB) was cannulated for IP measurement. A Doppler flow probe was placed around the renal arterial for renal blood flow (RBF) measurement. After the baseline MAP, HR, IP and RBF recordings for 15 min, GABA or bicuculline methiodate (BMI) or L-glutamate or kynurenic acid (KYN) or saline (vehicle) were bilaterally injected into the shell NAcc and the variables were measured for 30 min. Data are as mean ± SEM and submitted to Student́s t test. GABA injections into the shell NAcc evoked a significant fall in MAP and HR and increased IP and RC compared to saline. L-glutamate in the shell NAcc increased MAP, HR and IP and reduced RC. Injections of BMI and KYN elicited no changes in the variables recorded. Therefore, the GABAergic and glutamatergic transmissions in neurons in the shell NAcc are involved in the neural pathways responsible for the central cardiovascular control and UB regulation.
伏隔核（NAcc）的外壳投射到外侧视前区，该区域参与中央排尿控制，并接收来自涉及心血管控制的髓质区域的输入。我们研究了壳核中 GABA 能和谷氨酸能传递在膀胱内压（IP）和心血管控制中的作用。在实验前 7 天，将双侧导水管埋置在壳核中的雄性 Wistar 大鼠用 100% O 中的 2%异氟烷麻醉，并进行股动脉和静脉插管以分别记录平均动脉压（MAP）和心率（HR）以及药物输注。对膀胱（UB）进行插管以测量 IP。在肾动脉周围放置多普勒血流探头以测量肾血流量（RBF）。在记录基线 MAP、HR、IP 和 RBF 15 分钟后，将 GABA 或荷包牡丹碱甲碘酸盐（BMI）或 L-谷氨酸或犬尿氨酸（KYN）或生理盐水（载体）双侧注入壳核，并测量 30 分钟。数据为均值±SEM，并提交给学生 t 检验。与生理盐水相比，将 GABA 注入壳核会引起 MAP 和 HR 明显下降，而 IP 和 RC 增加。壳核中的 L-谷氨酸增加了 MAP、HR 和 IP，并减少了 RC。BMI 和 KYN 的注射未引起记录变量的变化。因此，壳核神经元中的 GABA 能和谷氨酸能传递参与了负责中央心血管控制和 UB 调节的神经通路。
