兔可渗透肌纤维中的磷酸盐爆发
Phosphate burst in permeable muscle fibers of the rabbit.
作者信息
Ferenczi M A
出版信息
Biophys J. 1986 Sep;50(3):471-7. doi: 10.1016/S0006-3495(86)83484-1.
Abstract
The transient kinetics of ATP hydrolysis in chemically skinned psoas muscle fibers of the rabbit have been measured. Muscles fibers in the rigor state (absence of nucleotide) were relaxed rapidly by the photochemical release of [2-3H]ATP from caged-ATP (P3-1-(2-nitro)phenylethyl[2-3H]adenosine 5'-triphosphate) in the absence of calcium ions. Rapid freezing of the fiber to stop hydrolysis, followed by analysis of the tritiated nucleotide content allowed the course of the hydrolysis to be determined. The timecourse of ATP hydrolysis was biphasic, with an initial rapid phase occurring at a rate of approximately 60 s-1 at 12 degrees C for fibers exposed to greater than 0.7 mM ATP. The amplitude of the rapid phase was as previously reported (Ferenczi, M. A., E. Homsher, and D. R. Trentham, 1984, J. Physiol. (Lond.)., 352:575-599).
摘要
已测定了兔化学去膜腰大肌纤维中ATP水解的瞬态动力学。在无钙离子的情况下，通过从笼形ATP（P3-1-(2-硝基)苯乙基[2-3H]腺苷5'-三磷酸）光化学释放[2-3H]ATP，使处于僵直状态（无核苷酸）的肌纤维迅速松弛。将纤维快速冷冻以停止水解，随后分析氚化核苷酸含量，从而确定水解过程。ATP水解的时间进程是双相的，对于暴露于大于0.7 mM ATP的纤维，在12℃下初始快速阶段以约60 s-1的速率发生。快速阶段的幅度如先前报道（费伦齐，M. A.，E. 霍姆斯赫，和D. R. 特伦瑟姆，1984，《生理学杂志》（伦敦），352:575 - 599）。
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