通过可激发的人工液膜识别手性的可能性。

A possibility to recognize chirality by an excitable artificial liquid membrane.

作者信息

Yoshikawa K, Omochi T, Matsubara Y, Kourai H

出版信息

Biophys Chem. 1986 Jul;24(2):111-9. doi: 10.1016/0301-4622(86)80004-7.

Abstract

Studies were made on oscillations across a liquid membrane consisting of an oil layer, nitrobenzene containing picric acid, between two aqueous layers: a solution of 1.5 M ethanol and 5 mM optically-active cationic detergent, the D- or L-form of N-alpha-methylbenzyl-N,N-dimethylmyristylammonium bromide, on the left and 0.1 M D- or L-form of various ligands, such as glucose, arabinose, alanine, glutamic acid, threonine, leucine, proline, or phenylalanine on the right. This system showed sustained rhythmic oscillations of electrical potential of 200-300 mV with intervals of the order of 1 min. The frequency of oscillations depended on the combination of chiralities of the detergent and ligand. This means that the two forms (D and L) of chiral ligands can be distinguished by differences in the electrical response of the liquid membrane.

摘要

对由油层、含苦味酸的硝基苯组成的液膜在两个水层之间的振荡进行了研究：左侧为1.5 M乙醇和5 mM旋光性阳离子洗涤剂（N-α-甲基苄基-N，N-二甲基肉豆蔻基溴化铵的D型或L型）的溶液，右侧为0.1 M各种配体（如葡萄糖、阿拉伯糖、丙氨酸、谷氨酸、苏氨酸、亮氨酸、脯氨酸或苯丙氨酸）的D型或L型。该系统显示出持续的有节奏的电位振荡，幅度为200 - 300 mV，间隔约为1分钟。振荡频率取决于洗涤剂和配体手性的组合。这意味着手性配体的两种形式（D型和L型）可以通过液膜电响应的差异来区分。