Bhavikatti Ayesha, Channigaramaiah Guruprasad, Chikkannaiah Panduranga, Venkataramanappa Srinivasamurthy
Department of Pathology, MS Ramaiah Medical College, Bengaluru, Karnataka, India.
Department of Pathology, ESIC MC and PGIMSR, Bengaluru, Karnataka, India.
J Lab Physicians. 2023 Jan 30;15(3):365-371. doi: 10.1055/s-0042-1760671. eCollection 2023 Sep.
Cancer stem cell markers are now being tried in various cancers as prognostic markers including GI cancer but these kinds of studies are sparse in Indian population. This study conducted over a period 50 months. Hematoxylin and eosin-stained slides were screened for grading of the tumor, extent of invasion of tumor, confirmation of metastasis, and staging was done. Immunohistochemical expression of CD44 was graded on the basis of percentage of tumor cells positive for staining. Statistical analysis was done and results were tabulated. : A total of 40 cases of GI cancer were studied. Ascending colon (37.5%) was the common site involved, 37 cases (92.5%) showed invasion beyond the muscularis externa. Most tumors were poorly differentiated (37.5%). Also, 50% of lymph nodes showed tumor deposits. The majority of the cases were in stage II (40%). There was a significant correlation between histopathological type of differentiation with lymph node metastasis and staging of tumor, lymph node metastasis also had significant association with staging. Grade 2, CD 44 expression was most common followed by Grade 3. Significant association was observed between histopathological differentiations of tumor with CD44 expression. Tumors that are invading beyond muscularis externa and lymph node-positive cases showed moderate to high CD44 expression. CD44 expression was significantly noted in poorly differentiated tumors. Increased expression was also noted in cases of tumors invading beyond muscularis externa and lymph node metastasis. Combination of CSC markers will increase the sensitivity and specificity and predict better overall survival in GI tumors.
癌症干细胞标志物目前正在各种癌症中作为预后标志物进行试验，包括胃肠道癌症，但这类研究在印度人群中较为稀少。 本研究历时50个月。对苏木精和伊红染色的切片进行筛查，以确定肿瘤分级、肿瘤浸润范围、转移确认，并进行分期。CD44的免疫组化表达根据染色阳性的肿瘤细胞百分比进行分级。进行了统计分析并将结果制成表格。 ：共研究了40例胃肠道癌症病例。升结肠（37.5%）是最常受累的部位，37例（92.5%）显示侵犯超过肌层。大多数肿瘤分化不良（37.5%）。此外，50%的淋巴结有肿瘤沉积。大多数病例处于II期（40%）。组织病理学分化类型与淋巴结转移和肿瘤分期之间存在显著相关性，淋巴结转移也与分期有显著关联。2级CD44表达最常见，其次是3级。肿瘤的组织病理学分化与CD44表达之间存在显著关联。侵犯超过肌层的肿瘤和淋巴结阳性病例显示中度至高度CD44表达。 CD44表达在分化不良的肿瘤中显著可见。在侵犯超过肌层和有淋巴结转移的肿瘤病例中也观察到表达增加。癌症干细胞标志物的联合使用将提高敏感性和特异性，并更好地预测胃肠道肿瘤的总体生存率。
