Fajami Zahra, Akbarin Mohammad Mehdi, Rafatpanah Houshang, Ramezani Samaneh, Rahimi Hossein, Rezaee Seyed Abdolrahim
Department of Modern Sciences and Technologies, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Inflammation and Inflammatory Diseases Research Centre, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
AIDS Res Hum Retroviruses. 2024 Mar;40(3):141-147. doi: 10.1089/AID.2023.0025. Epub 2023 Sep 25.
Adult T cell leukemia/lymphoma is a malignancy with a poor prognosis caused by human T lymphocyte virus type 1 (HTLV-1) infection. Tax and HBZ are two major viral proteins that may be involved in oncogenesis by disrupting apoptosis. Because Bcl-xL plays an integral role in the anti-apoptotic pathway, this study examines the interaction between host apoptosis and oncoproteins. We investigated 37 HTLV-1-infected individuals, including 18 asymptomatic and 19 adult T cell leukemia/lymphoma (ATLL) subjects. mRNA was extracted and converted to cDNA from peripheral blood mononuclear cells, and then gene expression was determined using TaqMan q-PCR. Moreover, the HTLV-1 proviral load (PVL) was also measured using a commercial absolute quantification kit (Novin Gene, Iran). Data analysis revealed that the mean of , , and PVL was significantly higher among the study groups (ATLL and carrier groups = .003, = .000, and = .002 respectively). There was no statistical difference in Bcl-xL gene expression between the study groups ( = .323). It is proposed that this anti-apoptotic pathway may not be directly involved in the development of ATLL lymphoma. , TAX, HBZ gene expression, and PVL can be utilized as prognostic markers.
成人T细胞白血病/淋巴瘤是一种由人类T淋巴细胞病毒1型（HTLV-1）感染引起的预后不良的恶性肿瘤。Tax和HBZ是两种主要的病毒蛋白，它们可能通过破坏细胞凋亡参与肿瘤发生。由于Bcl-xL在抗凋亡途径中起不可或缺的作用，本研究考察宿主细胞凋亡与癌蛋白之间的相互作用。我们调查了37名HTLV-1感染个体，包括18名无症状个体和19名成人T细胞白血病/淋巴瘤（ATLL）患者。从外周血单个核细胞中提取mRNA并转化为cDNA，然后使用TaqMan q-PCR测定基因表达。此外，还使用商业绝对定量试剂盒（伊朗Novin Gene公司）测量HTLV-1前病毒载量（PVL）。数据分析显示，研究组（ATLL组和携带者组）中 、 和PVL的平均值显著更高（分别为 = 0.003、 = 0.000和 = 0.002）。研究组之间Bcl-xL基因表达无统计学差异（ = 0.323）。有人提出，这种抗凋亡途径可能不直接参与ATLL淋巴瘤的发生。 、TAX、HBZ基因表达和PVL可作为预后标志物。
