衰老时中下丘脑基底部神经元中 MicroRNA-200a/b/c 的表达

Expression of MicroRNA-200a/b/c in the Mediobasal Hypothalamic Nuclei with Aging.

机构信息

Department of Pathophysiology, Yaroslavl State Medical University, ul. Revoliucionnaya 5, Yaroslavl, 150000, Russia.

Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, ul. Revoliucionnaya 5, Yaroslavl, 150000, Russia.

出版信息

Microrna. 2023;12(3):227-232. doi: 10.2174/2211536612666230810094531.

DOI:10.2174/2211536612666230810094531

PMID:37565555

Abstract

BACKGROUND

MicroRNAs (miRNAs) belong to small non-coding RNAs that coordinate the expression of cellular genes at the post-transcriptional level. The hypothalamus is a key regulator of homeostasis, biological rhythms and adaptation to different environmental factors. It also participates in the aging regulation. Variations in miRNA expression in the hypothalamus can affect the aging process.

OBJECTIVE

Our objective of this study is to examine the expression of miR-200a-3p, miR-200b-3p, miR-200c-3p in the dorsomedial (DMN), ventromedial (VMN) and arcuate (ARN) nuclei of the hypothalamus in male and female rats during aging.

METHODS

The expression of miR-200a-3p, miR-200b-3p, and miR-200c-3p in DMN, VMN and ARN was studied by qPCR-RT. The results were presented using the 2 algorithm.

RESULTS

The expression of miR-200a-3p, miR-200b-3p, miR-200c-3p microRNAs decreases with aging in the DMN of males and in the VMN of females. The level of miR-200b-3p expression decreased in aged males in the VMN and females in the DMN. The expression of miR-200c-3p declined in aged males in the ARN and in females in the DMN. The expression of miR-200a-3p, miR-200b-3p, and miR-200c-3p did not change in females in the ARN in aging.

CONCLUSION

We found a decrease in the expression of members of the miR-200a-3p, miR-200b-3p, and miR-200c-3p in the tuberal hypothalamic nuclei and their sex differences in aging rats.

摘要

背景

MicroRNAs (miRNAs) 属于小的非编码 RNA，在后转录水平上协调细胞基因的表达。下丘脑是内稳态、生物节律和适应不同环境因素的关键调节者。它还参与衰老的调节。下丘脑 miRNA 表达的变化会影响衰老过程。

目的

本研究的目的是检测雄性和雌性大鼠衰老过程中下丘脑背内侧核（DMN）、腹内侧核（VMN）和弓状核（ARN）中 miR-200a-3p、miR-200b-3p 和 miR-200c-3p 的表达。

方法

通过 qPCR-RT 研究 DMN、VMN 和 ARN 中 miR-200a-3p、miR-200b-3p 和 miR-200c-3p 的表达。结果采用 2 算法表示。

结果

在雄性 DMN 和雌性 VMN 中，miR-200a-3p、miR-200b-3p 和 miR-200c-3p 微 RNA 的表达随年龄增长而降低。VMN 中雄性和 DMN 中雌性的 miR-200b-3p 表达水平降低。在雄性的 ARN 和雌性的 DMN 中，miR-200c-3p 的表达下降。在衰老过程中，雌性的 ARN 中 miR-200a-3p、miR-200b-3p 和 miR-200c-3p 的表达没有变化。