Lie T, Gusberti F
Acta Odontol Scand. 1979;37(2):65-72. doi: 10.3109/00016357909027574.
Plaque formation on buccal tooth surfaces was studied by replica technique, consisting of impressions using low viscosity silicone impression materials and positive models produced in epoxy resins. Bacterial accumulation occurred near the cemento-enamel junction in 6-hr specimens, and subsequently expanded in a coronal direction. This development took place partly by extensions of single layers of bacteria, and partly by a pattern where the colonization was mostly restricted to vertical enamel cracks. Plaque accumulations were also frequently located in abrasion grooves and surface pits in the enamel, and prolific plaque areas were consistently surrounded by a monolayer of bacterial cells. Globular and hemispheric structures which occurred, especially on root surfaces immediately after cleaning, were probably artefacts caused by air bubbles or remaining moisture. In separate series of experiments it was demonstrated that improved reproduction of details from the plaque could be achieved by repeating the replicating procedure. The findings indicate that plaque formation starts by adsorption and proliferation of individual bacteria on tooth surfaces, and not by adsorption of aggregates of cells. Special attention should be directed against the problem of artefacts and moisture in replica studies of dental plaque.
采用复制技术研究颊侧牙面菌斑的形成，该技术包括使用低粘度硅橡胶印模材料制取印模以及用环氧树脂制作阳性模型。在6小时的标本中，细菌在牙骨质-釉质界附近积聚，随后向冠方扩展。这种发展部分是通过单层细菌的延伸，部分是通过一种模式，即菌落大多局限于垂直的釉质裂纹。菌斑积聚也经常位于釉质的磨损沟和表面凹坑中，大量菌斑区域始终被单层细菌细胞包围。尤其是在清洁后立即出现在根面上的球状和半球状结构，可能是由气泡或残留水分导致的假象。在单独的一系列实验中表明，通过重复复制过程可以更好地再现菌斑的细节。研究结果表明，菌斑形成始于单个细菌在牙面的吸附和增殖，而非细胞聚集体的吸附。在牙菌斑复制研究中，应特别关注假象和水分问题。
