使用 T 细胞转移、固有层制备和流式细胞术来描述小鼠小肠中的 CD4 T 细胞分化。

Characterizing CD4 T cell differentiation in mouse small intestine using T cell transfer, lamina propria preparation, and flow cytometry.

机构信息

Division of Gastroenterology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Department of Medicine, Section of Hematology-Medical Oncology, Boston University School of Medicine, Boston Medical Center, Boston, MA 02118, USA.

出版信息

STAR Protoc. 2023 Sep 15;4(3):102485. doi: 10.1016/j.xpro.2023.102485. Epub 2023 Aug 9.

DOI:10.1016/j.xpro.2023.102485

PMID:37566548

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10440592/

Abstract

Studying gene function in T cells is crucial for understanding physiology and disease pathogenesis. Here, we provide a protocol to examine the role of specific genes in CD4 T cell differentiation in the intestine. We describe steps for isolating naïve CD4 T cells from mouse spleens and transferring them to recipient mice. We detail procedures to isolate lamina propria cells and analyze CD4 T subsets using flow cytometry. This protocol is useful in the study of mucosal immune functions. For complete details on the use and execution of this protocol, please refer to Duan et al..

摘要

研究 T 细胞中的基因功能对于理解生理和疾病发病机制至关重要。本文提供了一种方案，用于研究肠道中特定基因在 CD4 T 细胞分化中的作用。我们描述了从小鼠脾脏中分离初始 CD4 T 细胞并将其转移到受体小鼠中的步骤。我们详细介绍了分离固有层细胞和使用流式细胞术分析 CD4 T 细胞亚群的程序。该方案可用于研究黏膜免疫功能。如需详细了解本方案的使用和执行，请参考 Duan 等人的研究。