Guan Chunmin, Wu Ying, Luo Lijuan, Qiao Fan, Zhu Jianhua, Lin Qinlu, Liu Chun
National Engineering Research Center for Rice and By-product Deep Processing, School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Int J Biol Macromol. 2023 Oct 1;250:126255. doi: 10.1016/j.ijbiomac.2023.126255. Epub 2023 Aug 9.
In this study, curcumin@high-pressure homogenization-soybean 7S protein/nanoparticles (CUR@HPH-7S-NPs) were prepared by an anti-solvent method. The physicochemical properties results showed at a CUR concentration of 4 mg/mL, CUR@HPH-7S-NPs had better size, encapsulation efficiency (EE), and zeta-potential values of 151.9 nm, 88.80 %, and -23.1 mV, respectively. Fourier transforms infrared (FTIR) and endogenous fluorescence spectroscopy results indicated CUR bound to HPH-7S through hydrophobic interactions, and the force between HPH-7S and CUR molecules was greater than that between untreated 7S protein and CUR. Furthermore, the pH stability results showed the size of CUR@HPH-7S-NPs was barely affected by pH away from adjacent area of the isoelectric point of 7S protein. The physical thermal stability and bio-accessibility results suggested that HPH-7S was more effective in delaying the degradation, had more physical thermal stability, and had a significant improvement in the bio-accessibility of CUR than that of untreated 7S protein. What's more, the antioxidant activity results showed at a CUR equivalent concentration of 40 μg/mL, the DPPH and ABTS radical scavenging activity of CUR@HPH-7S-NPs was 85.10 % and 96.64 %, respectively, both of which were significantly higher than that of free CUR. Finally, this study aimed to provide a theoretical basis for the delivery of other hydrophobic bioactive substances.
在本研究中，通过反溶剂法制备了姜黄素@高压均质化大豆7S蛋白/纳米颗粒（CUR@HPH-7S-NPs）。物理化学性质结果表明，在姜黄素浓度为4mg/mL时，CUR@HPH-7S-NPs具有更好的粒径、包封率（EE）和zeta电位值，分别为151.9nm、88.80%和-23.1mV。傅里叶变换红外光谱（FTIR）和内源荧光光谱结果表明，姜黄素通过疏水相互作用与HPH-7S结合，且HPH-7S与姜黄素分子之间的作用力大于未处理的7S蛋白与姜黄素之间的作用力。此外，pH稳定性结果表明，远离7S蛋白等电点相邻区域时，CUR@HPH-7S-NPs的粒径几乎不受pH影响。物理热稳定性和生物可及性结果表明，与未处理的7S蛋白相比，HPH-7S在延缓降解方面更有效，具有更高的物理热稳定性，且姜黄素的生物可及性有显著提高。更重要的是，抗氧化活性结果表明，在姜黄素等效浓度为40μg/mL时，CUR@HPH-7S-NPs的DPPH和ABTS自由基清除活性分别为85.10%和96.64%，均显著高于游离姜黄素。最后，本研究旨在为其他疏水性生物活性物质的递送提供理论依据。
