Atzpodien J, Gulati S C, Clarkson B D
Cancer Res. 1986 Oct;46(10):4892-5.
Various chemical compounds have been described to induce photosensitization of tumor cells resulting in cell death. We studied the effect of merocyanine-540 (MC-540) on both leukemic and normal bone marrow (BM) cells. Acute promyelocytic leukemia (HL-60) and common acute lymphoblastic leukemia antigen-positive non-T, non-B acute lymphoblastic leukemia (Reh) cell lines were incubated with MC-540 and simultaneously exposed to white light. Normal human BM and mixtures of leukemic cells with BM cells were treated under similar conditions. At constant illumination rates of 50,000 lx, significant (at least 4 to 5 logs) tumor cell destruction was obtained with MC-540 concentrations of 20 micrograms/ml or more for HL-60, and 10 micrograms/ml or more for Reh cells. Incubation of BM under equivalent conditions preserved 18.0% of granulocyte-macrophage colony-forming units and 14.2% of erythroid burst-forming units. Similar results were obtained when tumor cells were mixed with irradiated BM and then treated with MC-540. In summary, cell photosensitization with MC-540 has a selective cytotoxic effect towards leukemic cells and therefore may be useful for purging tumor cells from autologous BM.
已有多种化合物被描述可诱导肿瘤细胞发生光致敏作用，从而导致细胞死亡。我们研究了部花青-540（MC-540）对白血病细胞和正常骨髓（BM）细胞的影响。将急性早幼粒细胞白血病（HL-60）细胞系和普通急性淋巴细胞白血病抗原阳性的非T、非B急性淋巴细胞白血病（Reh）细胞系与MC-540一起孵育，并同时暴露于白光下。正常人类骨髓以及白血病细胞与骨髓细胞的混合物在相似条件下进行处理。在50,000勒克斯的恒定光照速率下，对于HL-60细胞，MC-540浓度为20微克/毫升或更高时，可导致显著（至少4至5个对数级）的肿瘤细胞破坏；对于Reh细胞，MC-540浓度为10微克/毫升或更高时，可导致显著肿瘤细胞破坏。在同等条件下对骨髓进行孵育，可保留18.0%的粒细胞-巨噬细胞集落形成单位和14.2%的红系爆式集落形成单位。当肿瘤细胞与经辐照的骨髓混合，然后用MC-540处理时，也获得了类似结果。总之，MC-540介导的细胞光致敏作用对白血病细胞具有选择性细胞毒性作用，因此可能有助于从自体骨髓中清除肿瘤细胞。
