Malo Nondita, Mazumder Debashish, Crawford Jagoda, Gadd Patricia, Gopi Karthik, Saintilan Neil, Sammut Jesmond
Centre for Ecosystem Science, School of Biological, Earth and Environmental Sciences, The University of New South Wales (UNSW), Sydney, NSW 2052, Australia.
Australian Nuclear Science and Technology Organisation (ANSTO), Locked Bag 2001, Sydney, NSW 2232, Australia.
Foods. 2023 Jul 28;12(15):2874. doi: 10.3390/foods12152874.
Seafood elemental profiling (SEP) is the quantification of a range of elements in seafood products and may serve in addressing questions of seafood provenance and quality. Traditional methods for analyzing soft tissue present several limitations for the industry-level use of SEP. Portable handheld X-ray fluorescence (pXRF) analysis is a promising alternative to conventional methods; however, its application for biological analysis has not been fully established. Intact giant tiger prawn () abdomens were analyzed with a Vanta M series XRF portable analyzer following a novel soft tissue protocol. Exploratory statistics (principal component analysis, nonmetric multidimensional scaling, and canonical discriminant analysis), as well as random forest models, have been implemented with pXRF profiles, yielding 81% accuracy when assigning the geographical origin of . The results of this study highlight that SEP via pXRF is a viable industry-level analysis, and its application will depend on improved instrument calibration to account for fluctuating wetness factors that are influenced by cooking, storage, and other pre- and post-harvest treatments.
海鲜元素剖析（SEP）是对海产品中一系列元素进行定量分析，可用于解决海产品来源和质量问题。传统的软组织分析方法在行业层面应用SEP时存在若干局限性。便携式手持式X射线荧光（pXRF）分析是传统方法的一种有前景的替代方法；然而，其在生物分析中的应用尚未完全确立。按照一种新颖的软组织检测方案，使用Vanta M系列XRF便携式分析仪对完整的斑节对虾腹部进行了分析。利用pXRF分析数据实施了探索性统计分析（主成分分析、非度量多维标度法和典型判别分析）以及随机森林模型，在确定斑节对虾地理来源时准确率达到81%。本研究结果表明，通过pXRF进行SEP是一种可行的行业层面分析方法，其应用将取决于改进仪器校准，以考虑受烹饪、储存以及其他收获前和收获后处理影响的湿度波动因素。
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