Jiménez-Ruiz Claudio A, de la Herrán Roberto, Robles Francisca, Navajas-Pérez Rafael, Cross Ismael, Rebordinos Laureana, Ruiz-Rejón Carmelo
Departamento de Genética, Facultad de Ciencias, Universidad de Granada, Avda. Fuentenueva s/n, 18071 Granada, Spain.
Área de Genética, Facultad de Ciencias del Mar y Ambientales, Instituto Universitario de Investigación Marina (INMAR), Universidad de Cádiz, 11510 Cádiz, Spain.
Animals (Basel). 2023 Jul 25;13(15):2399. doi: 10.3390/ani13152399.
The miR-430 microRNA family has been described in multiple fish species as one of the first microRNAs expressed by the zygote. It has been suggested that this family is implicated in maternal mRNA elimination, but may also play a role in steroidogenesis, sexual differentiation, and flatfish metamorphosis. The miR-430 sequences have been found in multiple-copy tandem clusters but evidence of their conservation outside of teleost fishes is scarce. In the present study, we have characterized the tandem repeats organization of these microRNAs in different fish species, both model and of interest in aquaculture. A phylogenetic analysis of this family has allowed us to identify that the miR-430 duplication, which took place before the Chondrostei and Neopterygii groups' divergence, has resulted in three variants ("a", "b", and "c"). According to our data, variant "b" is the most closely related to the ancestral sequence. Furthermore, we have detected isolated instances of the miR-430 repeat subunit in some species, which suggests that this microRNA family may be affected by DNA rearrangements. This study provides new data about the abundance, variability, and organization of the miR-430 family in fishes.
miR-430微小RNA家族在多种鱼类中被描述为合子表达的首批微小RNA之一。有人认为该家族与母源mRNA的消除有关，但也可能在类固醇生成、性别分化和比目鱼变态中发挥作用。已在多拷贝串联簇中发现miR-430序列，但在硬骨鱼之外其保守性的证据很少。在本研究中，我们对这些微小RNA在不同鱼类（包括模式鱼类和水产养殖中有研究价值的鱼类）中的串联重复序列组织进行了表征。对该家族的系统发育分析使我们能够确定，miR-430的复制发生在软骨硬鳞鱼和新鳍鱼群分化之前，产生了三个变体（“a”、“b”和“c”）。根据我们的数据，变体“b”与祖先序列关系最为密切。此外，我们在一些物种中检测到了miR-430重复亚基的孤立实例，这表明该微小RNA家族可能受到DNA重排的影响。本研究提供了有关鱼类中miR-430家族丰度、变异性和组织的新数据。
